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St8sia5 (NM_153124) Mouse Tagged ORF Clone
CAT＃: MR205839
- TrueORF®
St8sia5 (Myc-DDK-tagged) - Mouse ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5 (St8sia5), transcript variant 2
"NM_153124" in other vectors (3)
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CNY 3,330.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Siat8e; ST8SiaV |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR205839 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCTACGCAGACCCCTCCGCCAACAGAGATTTGTTGGGGAATCGAACTTTGCTCTTTATCTTCATCT GCGCCTTTGCCTTGGTGACCTTGCTTCAGCAGATCCTGTACAGCAAGAGCTACATCAAAAGGTACTTCGA GTTTTACAAGGAGCCTTTAGAGTTTAACTCCACGAGATGCCTGGAGCTGAGGCAGGAGATCCTGGAGGTG AAGGTGCTGTCCATGGTGAAGCAGTCTGAACTCTTCGAAAGGTGGAAGAGTCTCCAGATATGCAAATGGG CAATGGACACCGCGGAAGCCAGCCTCTTCAAGTCCACCTTGTCCAGGTGCTGCAATGCCCCCAGCTTTCT CTTCACCACCCAGAAGAACACCCCGGTGGAGACGAATCTCAGGTATGAGGTGGAATCCAGCGGCTTATAT CACATCGACCAGGAGATCTTCAAAATGTTTCCCAAGGAAATGCCCTACTACCGCTCTCAGTTTAAGAAGT GTGCTGTGGTGGGCAATGGCGGCATCTTGAAGAACAGCGGCTGCGGGAAGGAGATCAACAGTGCTGACTT TGTCTTCCGGTGCAACCTGCCCCCCATCTCAGGGATATATACCACAGATGCGGGAGAGAAGACAGATGTA GTCACCGTGAATCCCAGCATCATAATTGACAGGTTCCACAAGTTGGAGAAGTGGCGGCGGCCTTTCTTCA GCGTGCTGCAGAGGTACGAGAACGCCTCAGTGCTGCTGCCAGCGTTCTACAACGTCCGCAACACCCTGGT ATCCTTCCGAGTCAAATACATGCTGGACGACTTCCAGTCGCGGCAGCCGGTCTACTTCTTCCACCCGCAG TACCTGAGCAGTGTGTCGCGCTACTGGCTCAGCCTGGGTGTGCGCGCGCGGCGTATCAGCACCGGCCTCA TCCTGGTCACCGCGGCCCTGGAGCTCTGCGAGGAGGTGCACCTGTTCGGTTTCTGGGCCTTCCCCATGAA CCCCTCCGGCTTCTTCATCACGCACCATTACTATGACAACGTCAAGCCCAAGCCGGGTTTCCATGCTATG CCCTCGGAGATCTTCACCTTCCTGCGCATGCACAGCCGCGGCATCCTTCGCGTGCACACAGGCACCTGCA ATTGCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205839 protein sequence
Red=Cloning site Green=Tags(s) MRYADPSANRDLLGNRTLLFIFICAFALVTLLQQILYSKSYIKRYFEFYKEPLEFNSTRCLELRQEILEV KVLSMVKQSELFERWKSLQICKWAMDTAEASLFKSTLSRCCNAPSFLFTTQKNTPVETNLRYEVESSGLY HIDQEIFKMFPKEMPYYRSQFKKCAVVGNGGILKNSGCGKEINSADFVFRCNLPPISGIYTTDAGEKTDV VTVNPSIIIDRFHKLEKWRRPFFSVLQRYENASVLLPAFYNVRNTLVSFRVKYMLDDFQSRQPVYFFHPQ YLSSVSRYWLSLGVRARRISTGLILVTAALELCEEVHLFGFWAFPMNPSGFFITHHYYDNVKPKPGFHAM PSEIFTFLRMHSRGILRVHTGTCNCC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153124 |
| ORF Size | 1131 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153124.1 |
| RefSeq Size | 2006 bp |
| RefSeq ORF | 1131 bp |
| Locus ID | 225742 |
| MW | 43.8 kDa |
| Gene Summary | May be involved in the synthesis of gangliosides GD1c, GT1a, GQ1b and GT3 from GD1a, GT1b, GM1b and GD3 respectively.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201411 | St8sia5 (untagged) - Mouse ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5 (St8sia5), transcript variant 2, (10ug) |
CNY 2,000.00 |
|
| MR205839L3 | Lenti ORF clone of St8sia5 (Myc-DDK-tagged) - Mouse ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5 (St8sia5), transcript variant 2 |
CNY 5,230.00 |
|
| MR205839L4 | Lenti ORF clone of St8sia5 (mGFP-tagged) - Mouse ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5 (St8sia5), transcript variant 2 |
CNY 5,230.00 |
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