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Agpat3 (NM_053014) Mouse Tagged ORF Clone
CAT＃: MR205835
- TrueORF®
Agpat3 (Myc-DDK-tagged) - Mouse 1-acylglycerol-3-phosphate O-acyltransferase 3 (Agpat3)
ORF Plasmid: tGFP
"NM_053014" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW061257; AW493985; D10Jhu12e; lpaat3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR205835 representing NM_053014
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCTGCTTGCCTACCTGAAGACCCAGTTCGTGGTGCACCTGCTCATTGGCTTCGTCTTCGTGGTGA GCGGGTTAATTATCAACTTCACCCAGCTGTGCACACTGGCCCTCTGGCCCATCAGCAAGCACCTATACCG CCGTATCAACTGCCGCTTGGCCTACTCGCTCTGGAGCCAGCTGGTCATGCTCCTGGAGTGGTGGTCCTGC ACGGAGTGCACTCTCTTCACCGACCAGGCCACCGTGGACCACTTTGGGAAGGAGCATGTGGTTGTTATCC TCAACCACAACTTCGAGATCGACTTCCTTTGTGGGTGGACGATGTGCGAGCGGTTTGGCGTGCTGGGGAG CTCCAAGGTGCTGGCTAAGAGGGAGCTGCTGTGTGTGCCTCTCATCGGCTGGACGTGGTACTTCCTAGAG ATCGTATTCTGCAAACGGAAGTGGGAGGAAGACCGGGACACCGTCATAGAGGGCCTGAGGCGCTTAGCTG ACTACCCAGAGTACATGTGGTTTCTCCTGTACTGCGAAGGAACACGCTTCACAGAGACCAAGCATCGCAT CAGCATGGAGGTGGCTGCCTCCAAGGGGCTGCCCCCACTCAAGTACCACCTGCTGCCCCGGACCAAGGGC TTTACCACGGCAGTCCAGTGCCTCCGGGGGACAGTTGCAGCTATCTATGACGTGACCCTGAACTTCAGAG GGAACAAGAACCCATCTCTACTGGGGATCCTGTATGGGAAGAAATATGAGGCAGACATGTGTGTGAGGAG GTTCCCCCTGGAAGACATCCCAGCAGATGAGACCAGCGCCGCCCAGTGGCTTCACAAGCTGTACCAGGAG AAGGATGCCCTGCAAGAGATGTACAAGCAGAAGGGTGTATTCCCAGGGGAGCAGTTCAAGCCTGCCCGAA GGCCGTGGACCCTCCTGAACTTCCTGTGCTGGGCCACCATCCTCCTCTCACCCCTCTTCAGCTTCGTCCT GGGTGTCTTTGCCAGTGGATCCCCGCTCCTCATCCTGACGTTCTTGGGGTTCGTGGGAGCAGCTTCCTTC GGAGTCCGGAGACTGATAGGAGTGACTGAGATAGAGAAAGGCTCCAGCTATGGCAACCAAGAGCTTAAGA AAAAGGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205835 representing NM_053014
Red=Cloning site Green=Tags(s) MGLLAYLKTQFVVHLLIGFVFVVSGLIINFTQLCTLALWPISKHLYRRINCRLAYSLWSQLVMLLEWWSC TECTLFTDQATVDHFGKEHVVVILNHNFEIDFLCGWTMCERFGVLGSSKVLAKRELLCVPLIGWTWYFLE IVFCKRKWEEDRDTVIEGLRRLADYPEYMWFLLYCEGTRFTETKHRISMEVAASKGLPPLKYHLLPRTKG FTTAVQCLRGTVAAIYDVTLNFRGNKNPSLLGILYGKKYEADMCVRRFPLEDIPADETSAAQWLHKLYQE KDALQEMYKQKGVFPGEQFKPARRPWTLLNFLCWATILLSPLFSFVLGVFASGSPLLILTFLGFVGAASF GVRRLIGVTEIEKGSSYGNQELKKKE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053014 |
| ORF Size | 1128 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_053014.3, NP_443747.2 |
| RefSeq Size | 3439 bp |
| RefSeq ORF | 1131 bp |
| Locus ID | 28169 |
| UniProt ID | Q9D517 |
| MW | 43.7 kDa |
| Gene Summary | Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (PubMed:15367102). Acts on LPA containing saturated or unsaturated fatty acids C16:0-C20:4 at the sn-1 position using C18:1, C20:4 or C18:2-CoA as the acyl donor (By similarity). Also acts on lysophosphatidylcholine, lysophosphatidylinositol and lysophosphatidylserine using C18:1 or C20:4-CoA (By similarity). Has a preference for arachidonoyl-CoA as a donor (PubMed:19114731). Has also a modest lysophosphatidylinositol acyltransferase (LPIAT) activity, converts lysophosphatidylinositol (LPI) into phosphatidylinositol (PubMed:19114731).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209809 | Agpat3 (untagged) - Mouse 1-acylglycerol-3-phosphate O-acyltransferase 3 (Agpat3), (10ug) |
CNY 3,990.00 |
|
| MG205835 | Agpat3 (tGFP-tagged) - Mouse 1-acylglycerol-3-phosphate O-acyltransferase 3 (Agpat3) |
CNY 3,710.00 |
|
| MR205835L3 | Lenti ORF clone of Agpat3 (Myc-DDK-tagged) - Mouse 1-acylglycerol-3-phosphate O-acyltransferase 3 (Agpat3) |
CNY 5,230.00 |
|
| MR205835L4 | Lenti ORF clone of Agpat3 (mGFP-tagged) - Mouse 1-acylglycerol-3-phosphate O-acyltransferase 3 (Agpat3) |
CNY 5,230.00 |
