United States
Germany
Japan
United Kingdom
China
Fancl (BC032876) Mouse Tagged Lenti ORF Clone
CAT＃: MR205825L3
- LentiORF®
Lenti ORF clone of Fancl (Myc-DDK-tagged) - Mouse Fanconi anemia, complementation group L (cDNA clone MGC:41031 IMAGE:1282316)
"BC032876" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 4,090.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2010322C19Rik; AW554273; B230118H11Rik; gcd; Phf9; Pog |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR205825).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC032876 |
| ORF Size | 1125 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC032876.1 |
| RefSeq Size | 1668 bp |
| RefSeq ORF | 1127 bp |
| Locus ID | 67030 |
| Gene Summary | This gene encodes the complementation group L subunit of the multimeric Fanconi anemia (FA) nuclear complex composed of proteins encoded by over ten Fanconi anemia complementation (FANC) group genes. The FA complex is necessary for protection against DNA damage. This gene product, an E3 ubiquitin ligase, catalyzes and is required for the monoubiquitination of the protein encoded by the Fanconi anemia, complementation group D2 gene, a critical step in the FA pathway (PMID: 12973351, 21229326). In mouse, mutations of this E3 ubiquitin ligase gene can lead to infertility in adult males and females, and a deletion of this gene can cause embryonic lethality in some genetic backgrounds. A pseudogene of this gene has been identified on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG205825 | Fancl (tGFP-tagged) - Mouse Fanconi anemia, complementation group L (cDNA clone MGC:41031 IMAGE:1282316) |
CNY 2,380.00 |
|
| MR205825 | Fancl (Myc-DDK-tagged) - Mouse Fanconi anemia, complementation group L (cDNA clone MGC:41031 IMAGE:1282316) |
CNY 3,656.00 |
|
| MR205825L4 | Lenti ORF clone of Fancl (mGFP-tagged) - Mouse Fanconi anemia, complementation group L (cDNA clone MGC:41031 IMAGE:1282316) |
CNY 4,090.00 |
