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Brap (BC038490) Mouse Tagged ORF Clone
CAT＃: MR205796
- TrueORF®
Brap (Myc-DDK-tagged) - Mouse BRCA1 associated protein (cDNA clone MGC:47099 IMAGE:3599718)
ORF Plasmid: tGFP
"BC038490" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 3010002G07Rik; BRAP2; IMP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR205796 representing BC038490
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTGCAATGGCCGCCAGTTCAACTCAATCGAAGATGACGTCTGCCAGCTGGTCTATGTGGAAAGGG CTGAAGTGCTGAAATCTGAAGATGGCGCCAGCCTCCCCGTGATGGACCTGACGGAGCTGCCCAAGTGCAC TGTGTGTCTGGAGCGGATGGACGAGTCTGTGAATGGCATCCTCACCACCCTCTGCAACCACAGCTTCCAT AGTCAGTGTCTGCAGCGGTGGGATGACACCACGTGTCCTGTGTGCCGATACTGTCAAACGCCAGAGCCAG TGGAAGAAAACAAATGTTTTGAGTGTGGTGTCCAGGAAAACCTCTGGATTTGTTTAATATGCGGCCACAT AGGCTGTGGGCGGTACGTGAGTCGGCATGCTTACAAGCACTTTGAGGAGACCCAGCACACATACGCCATG CAGCTCACCAACCATCGAGTCTGGGACTATGCTGGAGATAATTATGTCCATCGACTGGTTGCAAGCAAGA CGGATGGAAAGATCGTTCAGTACGAGTGTGAGGGCGACACCTGCCAGGAAGAGAAGATAGATGCCTTACA GTTAGAGTACTCGTACCTGTTGACAAGCCAGCTGGAATCGCAGCGGATATACTGGGAGAACAAAATCGTG CGCATAGAGAAGGACACGGCAGAGGAGATTAACAACATGAAGACCAAGTTTAAAGAGACCATCGAGAAGT GTGACAGCCTGGAGCTCAGGCTCAGTGACCTCCTGAAGGAGAAGCAGTCTGTGGAAAGGAAGTGTACCCA GCTGAACACCAGAGTGGCCAAGCTCAGCACGGAGCTGCAGGAGGAGCAGGAGCTGAACAAGTGTCTGCGC GCCAACCAGCTGGTGCTGCAGAACCAGCTCAAGGAGGAGGAGAAGCTGCTGAAGGAGACCTGTGCCCAGA AAGACCTGCAGATCACCGAGATCCAGGAGCAGCTGCGCGATGTCATGTTCTACCTGGAGACACAGCAGCA GATCAGCCACCTGCCTGCGGAGACGAGGCAGGAGATCCAGGAAGGCCAGATCAACATCGCCATGGCCTCA GCGCCCAACCCACCCTCTTCCGGGGCCGGTGGGAAGCTGCAGTCCAGAAAGGGCCGCAGCAAGAGGGGCA AG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205796 representing BC038490
Red=Cloning site Green=Tags(s) MACNGRQFNSIEDDVCQLVYVERAEVLKSEDGASLPVMDLTELPKCTVCLERMDESVNGILTTLCNHSFH SQCLQRWDDTTCPVCRYCQTPEPVEENKCFECGVQENLWICLICGHIGCGRYVSRHAYKHFEETQHTYAM QLTNHRVWDYAGDNYVHRLVASKTDGKIVQYECEGDTCQEEKIDALQLEYSYLLTSQLESQRIYWENKIV RIEKDTAEEINNMKTKFKETIEKCDSLELRLSDLLKEKQSVERKCTQLNTRVAKLSTELQEEQELNKCLR ANQLVLQNQLKEEEKLLKETCAQKDLQITEIQEQLRDVMFYLETQQQISHLPAETRQEIQEGQINIAMAS APNPPSSGAGGKLQSRKGRSKRGK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC038490 |
| ORF Size | 1122 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC038490.1 |
| RefSeq Size | 3135 bp |
| RefSeq ORF | 1124 bp |
| Locus ID | 72399 |
| MW | 115 kDa |
| Gene Summary | Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218446 | Brap (untagged) - Mouse BRCA1 associated protein (cDNA clone MGC:47099 IMAGE:3599718), (10ug) |
CNY 6,940.00 |
|
| MG205796 | Brap (tGFP-tagged) - Mouse BRCA1 associated protein (cDNA clone MGC:47099 IMAGE:3599718) |
CNY 2,280.00 |
|
| MR205796L3 | Lenti ORF clone of Brap (Myc-DDK-tagged) - Mouse BRCA1 associated protein (cDNA clone MGC:47099 IMAGE:3599718) |
CNY 3,990.00 |
|
| MR205796L4 | Lenti ORF clone of Brap (mGFP-tagged) - Mouse BRCA1 associated protein (cDNA clone MGC:47099 IMAGE:3599718) |
CNY 3,990.00 |
