United States
Germany
Japan
United Kingdom
China
Cntfr (NM_016673) Mouse Tagged ORF Clone
CAT＃: MR205731
- TrueORF®
Cntfr (Myc-DDK-tagged) - Mouse ciliary neurotrophic factor receptor (Cntfr), transcript variant 1
"NM_016673" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Cntf; Cntfralpha |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR205731 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCTTCTGTCCCATGGGCCTGCTGTGCTGTGCTTGCCGCTGCCGCCGCCGCTGTCTACACGCAGA AACACAGTCCACAGGAGGCACCCCACGTGCAGTATGAGCGTCTGGGCGCAGATGTGACGCTGCCGTGTGG GACAGCGAGCTGGGACGCAGCTGTGACATGGAGGGTAAACGGGACAGATCTGGCCCCTGACCTGCTCAAC GGCTCTCAGCTGATACTGCGAAGCTTAGAACTGGGCCACAGTGGCCTATACGCCTGTTTCCACCGTGACT CCTGGCACCTGCGCCACCAAGTCCTTCTACATGTGGGTTTGCCGCCGCGGGAGCCTGTGCTCAGCTGCCG CTCCAACACTTACCCCAAGGGCTTCTACTGCAGCTGGCACCTGCCCACCCCCACCTACATCCCCAATACC TTCAATGTGACTGTGCTGCATGGCTCCAAAATTATGGTCTGTGAGAAGGACCCAGCCCTCAAGAACCGCT GCCACATTCGGTACATGCACCTGTTCTCAACCATCAAGTACAAGGTCTCCATAAGTGTCAGCAACGCCTT GGGTCACAACACTACGGCCATCACCTTTGACGAATTCACCATTGTGAAGCCTGATCCTCCAGAAAACGTG GTGGCCCGGCCAGTGCCCAGCAACCCCCGTCGGCTGGAGGTGACATGGCAGACACCCTCAACTTGGCCCG ACCCTGAATCCTTTCCTCTCAAGTTCTTCCTGCGCTACCGGCCTCTCATCCTGGACCAATGGCAGCATGT GGAGCTCTCGGATGGCACAGCACACACCATCACAGATGCCTACGCAGGGAAGGAATACATCATCCAGGTG GCAGCCAAGGACAATGAGATTGGGACATGGAGTGACTGGAGCGTGGCTGCTCACGCCACACCCTGGACTG AGGAACCTCGGCATCTCACAACAGAAGCCCAGGCCCCCGAGACCACGACCAGCACCACCAGCTCATTGGC ACCCCCACCCACCACGAAGATCTGTGACCCTGGGGAGCTTGGCAGCGGCGGAGGACCCTCCATACTCTTC TTGACCAGTGTCCCAGTCACTCTGGTCCTGGCTGCCGCTGCTGCCACAGCCAACAATCTCCTGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205731 protein sequence
Red=Cloning site Green=Tags(s) MAASVPWACCAVLAAAAAAVYTQKHSPQEAPHVQYERLGADVTLPCGTASWDAAVTWRVNGTDLAPDLLN GSQLILRSLELGHSGLYACFHRDSWHLRHQVLLHVGLPPREPVLSCRSNTYPKGFYCSWHLPTPTYIPNT FNVTVLHGSKIMVCEKDPALKNRCHIRYMHLFSTIKYKVSISVSNALGHNTTAITFDEFTIVKPDPPENV VARPVPSNPRRLEVTWQTPSTWPDPESFPLKFFLRYRPLILDQWQHVELSDGTAHTITDAYAGKEYIIQV AAKDNEIGTWSDWSVAAHATPWTEEPRHLTTEAQAPETTTSTTSSLAPPPTTKICDPGELGSGGGPSILF LTSVPVTLVLAAAAATANNLLI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016673 |
| ORF Size | 1119 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016673.2, NP_057882.2 |
| RefSeq Size | 2000 bp |
| RefSeq ORF | 1119 bp |
| Locus ID | 12804 |
| UniProt ID | O88507 |
| MW | 40.8 kDa |
| Gene Summary | This gene encodes the alpha subunit of the ciliary neurotrophic factor (CNTF) receptor that triggers the assembly of a trimolecular complex upon binding to CNTF, and initiate a downstream signaling process. The encoded preproprotein undergoes proteolytic processing to generate a glycosylphosphatidylinositol-linked cell surface protein. Mice lacking the encoded protein die shortly after birth and exhibit a reduction of motoneuron number at birth. The transgenic disruption of this gene specifically in the skeletal muscle followed by a peripheral nerve lesion impairs motor neuron axonal regeneration across the lesion site. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205483 | Cntfr (untagged) - Mouse ciliary neurotrophic factor receptor (Cntfr), transcript variant 1, (10ug) |
CNY 3,656.00 |
|
| MR205731L3 | Lenti ORF clone of Cntfr (Myc-DDK-tagged) - Mouse ciliary neurotrophic factor receptor (Cntfr), transcript variant 1 |
CNY 5,230.00 |
|
| MR205731L4 | Lenti ORF clone of Cntfr (mGFP-tagged) - Mouse ciliary neurotrophic factor receptor (Cntfr), transcript variant 1 |
CNY 5,230.00 |
