Fbxo28 (NM_175127) Mouse Tagged ORF Clone

CAT#: MR205671

  • TrueORF®

Fbxo28 (Myc-DDK-tagged) - Mouse F-box protein 28 (Fbxo28)

ORF Plasmid: DDK tGFP



  "NM_175127" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 4833428J17Rik; 5730505P19Rik; D1Ertd578e; Fbx28; mKIAA0483
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR205671 representing NM_175127
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGGCGTCTGAGGAGCGGATGGCTGAGGAAGGAGGCGGCGGCCACGGCGACGGCGGCTCCTGTT
CGGCTGCCGGCTCCGCTCAGCGACAGCCCCCGGCGCCCCCGTCGCAGGCCCCGCCGCCCGGGTCCCAGGC
ACCCGCCGCGCCCGCGCTGGCACCGGACCACCTGCCCCAAAACAACACACTGGTGGCGCTGCCCATCGTA
GCCATCGAGAACATCCTCAGCTTTATGTCCTACGACGAAATTAGCCAGCTCCGCCTGGTTTGTAAGAGAA
TGGACCTGGTCTGTCAGAGAATGTTGAATCAGGGGTTTCTGAAAGTGGAGAGGTTCCATAACCTGTGTCA
GAAGCAAGTTAAAGCACAGCTACCGAGGAGAGAGTCAGAAAGAAGAAACCATTCTTTAGCTCGTCATGCA
GACATCCTTGCCGCTGTGGAAACGAGGTTGTCACTGTTGAATATGACCTTCATGAAGTATGTGGATTCCA
ACCTCTGTTGCTTCATCCCAGGAAAGGTGATTGATGAGATTTACCGTGTACTGAGATATGTCAATTCTAC
CAGAGCCCCTCAGCGAGCTCACGAGGTGCTGCAGGAGTTACGGGATATCTCCTCCATGGCGATGGAGTAC
TTTGATGAGAAGATTGTTCCAATTTTGAAGAGGAAATTGCCAGGATCAGATGTGTCAGGAAGACTCATGG
GCTCTCCTCCAGTTCCCGGGCCATCCGCAGCCCTGACAACAATGCAGCTCTTCTCCAAGCAAAACCCGTC
CAGACAAGAGGTTACCAAACTGCAGCAGCAGGTCAGAACCAACGGCGCTGGCGTGACTGTCCTCAGGCGT
GAGATTTCTGAGCTTCGCACCAAAGTGCAAGAGCAGCAGAAACAGCTTCAAGACCAAGACCAGAAACTGC
TCGAGCAGACCCAGATCATAGGCGAACAGAACGCACGGCTGGCAGAGCTGGAACGCAAGCTCCGGGAAGT
CATGGAAAGTGCAGTAGGGACCTCCTCAGGGTCTGGGCAGAGTGAGGAGTCCCCTCGGAAACGACGGAAG
GCAACGGAAGCCATAGACTCTCTTAGGAAATCCAAACGACTTCGGAATAGGAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR205671 representing NM_175127
Red=Cloning site Green=Tags(s)

MAAASEERMAEEGGGGHGDGGSCSAAGSAQRQPPAPPSQAPPPGSQAPAAPALAPDHLPQNNTLVALPIV
AIENILSFMSYDEISQLRLVCKRMDLVCQRMLNQGFLKVERFHNLCQKQVKAQLPRRESERRNHSLARHA
DILAAVETRLSLLNMTFMKYVDSNLCCFIPGKVIDEIYRVLRYVNSTRAPQRAHEVLQELRDISSMAMEY
FDEKIVPILKRKLPGSDVSGRLMGSPPVPGPSAALTTMQLFSKQNPSRQEVTKLQQQVRTNGAGVTVLRR
EISELRTKVQEQQKQLQDQDQKLLEQTQIIGEQNARLAELERKLREVMESAVGTSSGSGQSEESPRKRRK
ATEAIDSLRKSKRLRNRK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_175127
ORF Size 1104 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_175127.2, NP_780336.1
RefSeq Size 4932 bp
RefSeq ORF 1107 bp
Locus ID 67948
UniProt ID Q8BIG4
MW 41.4 kDa
Gene Summary Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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