Tssk1 (NM_009435) Mouse Tagged ORF Clone
CAT#: MR205606
- TrueORF®
Tssk1 (Myc-DDK-tagged) - Mouse testis-specific serine kinase 1 (Tssk1)
ORF Plasmid: tGFP
"NM_009435" in other vectors (4)
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CNY 3,656.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Stk22a; TSK-1; Tsk1; Tssk; Tssk1b |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR205606 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGACGCTGCCGTCCTCAAGCGACGAGGCTACATCATGGGGATAAACTTGGGAGAGGGCTCATATG CCAAAGTCAAATCCGCTTACTCTGAGCGCCTAAAGTTCAACGTGGCGGTCAAGATCATCGATCGCAAGAA AGCCCCCTCAGACTTCCTGGAGAAATTCCTTCCCCGGGAGATTGAGATTCTGGCCATGCTAAACCACCGC TCTATTGTCAAGACCTATGAGATCTTTGAGACATCAGATGGCAAGGTCTACATTGTCATGGAGCTTGGGG TCCAGGGTGACCTCCTCGAGTTCATCAAAACCAGAGGAGCCCTGCAAGAGGATGATGCTCGAAAGAAGTT CCACCAGCTCTCCTCGGCCATCAAGTACTGCCACGACCTGGATGTCGTCCACCGAGACCTCAAATGTGAG AACCTTCTGCTTGACAAGGACTTCAACATCAAACTGTCTGACTTCGGCTTCTCCAAGCGCTGCTTGCGGG ATGATAGTGGTCGACTGATATTAAGCAAGACCTTCTGTGGGTCAGCAGCTTATGCGGCCCCCGAGGTGCT GCAGGGCATCCCCTACCAGCCCAAGGTGTATGACATCTGGAGCCTGGGTGTGATTCTCTACATCATGGTC TGTGGCTCCATGCCCTACGATGACTCCAACATCAAAAAGATGCTGCGCATCCAGAAGGAGCACCGTGTCA ACTTTCCACGCTCTAAACACCTGACTGGTGAGTGCAAGGACCTCATCTACAGAATGTTACAGCCAGATGT CAACCGCCGGCTGCACATCGATGAGATCCTTAACCACTGCTGGGTGCAGCCCAAGGCACGAGGTCTGTCC TCTGGAGCCATCAACAAGGAGGGGGAAAGCTCCCGGGCCACTGAGCCCTCATGGATCCCTGAACCTGGTG CTGATAAGAAGTCTGCCACCAAGTTGGAGCCTAGGGAAGAGGCACGATCTGAGGCCCGGTCTGAGTCAAA ACCCCAGGAAGATACTTTGCAGGTGGTCAGGCAGTCAGAGAACGTGGGCCTCAGCAGCGAACTGAACAGG GACACAGAGGAAGGGCACCCCCAACAGCCTTCAGAGACACATACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205606 protein sequence
Red=Cloning site Green=Tags(s) MDDAAVLKRRGYIMGINLGEGSYAKVKSAYSERLKFNVAVKIIDRKKAPSDFLEKFLPREIEILAMLNHR SIVKTYEIFETSDGKVYIVMELGVQGDLLEFIKTRGALQEDDARKKFHQLSSAIKYCHDLDVVHRDLKCE NLLLDKDFNIKLSDFGFSKRCLRDDSGRLILSKTFCGSAAYAAPEVLQGIPYQPKVYDIWSLGVILYIMV CGSMPYDDSNIKKMLRIQKEHRVNFPRSKHLTGECKDLIYRMLQPDVNRRLHIDEILNHCWVQPKARGLS SGAINKEGESSRATEPSWIPEPGADKKSATKLEPREEARSEARSESKPQEDTLQVVRQSENVGLSSELNR DTEEGHPQQPSETHT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_009435 |
ORF Size | 1098 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009435.2, NP_033461.2 |
RefSeq Size | 1466 bp |
RefSeq ORF | 1098 bp |
Locus ID | 22114 |
UniProt ID | Q61241 |
MW | 41.6 kDa |
Gene Summary | Testis-specific serine/threonine-protein kinase required during spermatid development (PubMed:20053632, PubMed:23599433). Phosphorylates 'Ser-281' of TSKS (PubMed:20053632). Involved in the late stages of spermatogenesis, during the reconstruction of the cytoplasm (PubMed:20053632). During spermatogenesis, required for the transformation of a ring-shaped structure around the base of the flagellum originating from the chromatoid body (PubMed:20053632).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209597 | Tssk1 (untagged) - Mouse testis-specific serine kinase 1 (Tssk1), (10ug) |
CNY 3,990.00 |
|
MG205606 | Tssk1 (tGFP-tagged) - Mouse testis-specific serine kinase 1 (Tssk1) |
CNY 3,710.00 |
|
MR205606L3 | Lenti ORF clone of Tssk1 (Myc-DDK-tagged) - Mouse testis-specific serine kinase 1 (Tssk1) |
CNY 5,230.00 |
|
MR205606L4 | Lenti ORF clone of Tssk1 (mGFP-tagged) - Mouse testis-specific serine kinase 1 (Tssk1) |
CNY 5,230.00 |