Crlf2 (NM_016715) Mouse Tagged ORF Clone

CAT#: MR205490

  • TrueORF®

Crlf2 (Myc-DDK-tagged) - Mouse cytokine receptor-like factor 2 (Crlf2), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_016715" in other vectors (5)

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Mouse Protein / Lysate Request

CNY 2,850.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal TSLP Receptor Antibody
    • 100 ug

CNY 5,808.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms CRLM2; Ly114; Tpte2; Tslpr
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR205490 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCATGGGCACTCGCGGTCATCCTCCTGCCTCGGCTCCTTGCGGCGGCAGCGGCGGCGGCGGCGGTGA
CGTCACGGGGTGATGTCACAGTCGTCTGCCATGACCTGGAGACGGTGGAGGTCACGTGGGGCTCGGGCCC
CGACCACCACAGCGCCAACTTGAGCCTGGAGTTCCGTTATGGTACTGGCGCCCTGCAACCCTGCCCGCGA
TATTTCCTGTCCGGCGCTGGTGTCACTTCCGGGTGCATCCTCCCCGCGGCGAGGGCGGGGCTGCTGGAGC
TGGCACTGCGCGACGGAGGCGGGGCCATGGTGTTTAAGGCTAGGCAGCGCGCGTCCGCCTGGCTGAAGCC
CCGCCCACCTTGGAATGTGACGCTGCTCTGGACACCAGACGGGGACGTGACTGTCTCCTGGCCTGCCCAC
TCCTACCTGGGCCTGGACTACGAGGTGCAGCACCGGGAGAGCAATGACGATGAGGACGCCTGGCAGACGA
CCTCAGGGCCCTGCTGTGACTTGACAGTGGGCGGGCTCGACCCCGCGCGCTGCTATGACTTCCGGGTTCG
GGCGTCGCCCCGGGCCGCGCACTATGGCCTGGAGGCGCAGCCTAGCGAGTGGACAGCGGTGACAAGGCTT
TCCGGGGCAGCATCCGCGGCCTCCTGTACCGCAAGCCCCGCCCCATCCCCGGCCCTGGCCCCGCCCCTCC
TGCCCCTGGGCTGCGGCCTAGCAGCGCTGCTGACACTGTCCCTGCTCCTGGCCGCCCTGAGGCTTCGCAG
GGTGAAAGATGCGCTGCTGCCCTGCGTCCCTGACCCCAGCGGCTCCTTCCCTGGACTCTTTGAGAAGCAT
CACGGGAACTTCCAGGCCTGGATTGCGGACGCCCAGGCCACAGCCCCGCCAGCCAGGACCGAGGAGGAAG
ATGACCTCATCCACCCCAAGGCTAAGAGGGTGGAGCCCGAGGATGGCACCTCCCTCTGCACCGTGCCAAG
GCCACCCAGCTTCGAGCCAAGGGGGCCGGGAGGCGGGGCCATGGTGTCAGTGGGCGGGGCCACGTTCATG
GTGGGCGACAGCGGCTACATGACCCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR205490 protein sequence
Red=Cloning site Green=Tags(s)

MAWALAVILLPRLLAAAAAAAAVTSRGDVTVVCHDLETVEVTWGSGPDHHSANLSLEFRYGTGALQPCPR
YFLSGAGVTSGCILPAARAGLLELALRDGGGAMVFKARQRASAWLKPRPPWNVTLLWTPDGDVTVSWPAH
SYLGLDYEVQHRESNDDEDAWQTTSGPCCDLTVGGLDPARCYDFRVRASPRAAHYGLEAQPSEWTAVTRL
SGAASAASCTASPAPSPALAPPLLPLGCGLAALLTLSLLLAALRLRRVKDALLPCVPDPSGSFPGLFEKH
HGNFQAWIADAQATAPPARTEEEDDLIHPKAKRVEPEDGTSLCTVPRPPSFEPRGPGGGAMVSVGGATFM
VGDSGYMTL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016715
ORF Size 1080 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_016715.3
RefSeq Size 1338 bp
RefSeq ORF 1080 bp
Locus ID 57914
UniProt ID Q8CII9
MW 37.8 kDa
Gene Summary Receptor for thymic stromal lymphopoietin (TSLP). Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2. Implicated in the development of the hematopoietic system.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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