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Prkx (NM_016979) Mouse Tagged ORF Clone
CAT＃: MR205408
- TrueORF®
Prkx (Myc-DDK-tagged) - Mouse protein kinase, X-linked (Prkx)
ORF Plasmid: tGFP
"NM_016979" in other vectors (4)
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CNY 2,850.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Pkare; PKX1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR205408 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCGCCCGCTGGTGCCGCGGCGACCGTAAAAGATCCAGACCATGACCCCGTGAAGACCAAGGTCT CGGCGCCCGCCGCGGACCCCAAACCCAGGACATCGTCACAGAAGGCGGGGCACTCGTTACAAGATTGGGA CACCATAGCCACCGTGGGCACTGGGACCTTTGGCCGTGTAAACCTGGTGAAGGAGAAGACAGGCAGGCAA TACTGTGCCTTGAAGATTATGAGTATCCCAGATGTCATCCGCCTGAAACAGGAACAGCATGTGCAGAATG AGAAAGCAGTCCTGAAGGAAATAAACCATCCCTTCCTCATTAAACTGCTTTGGACAGGCCATGACAACCG CTTCCTATACATGTTGATGGAGTTCGTGCCTGGTGGTGAGCTCTTCACCTACCTGCGTAATCGTGGCCGA TTCTCCTCAGTTGCTTCAGTCTTCTACGCCACAGAGATTGTGTGTGCCATAGAATATCTACACTCAAAGG AAATCGTATACAGGGACCTGAAGCCTGAAAACATCCTTCTAGACAGGGAAGGACATATTAAACTGACAGA CTTCGGCTTCGCCAAGAAACTGGTGGATAGGACATGGACTCTCTGTGGGACACCAGAATATCTCGCCCCA GAAGTCATTCAGAGCAAAGGTCATGGAAGGGCTGTGGACTGGTGGGCACTGGGCATTCTTATATTCGAGA TGCTGTCTGGGTTTCCCCCATTTTTCGATGACAACCCGTTTGGGATTTACCAGAAAATCCTTGCATGCAA AATAGATTTCCCCAGACAATTGGATTTCACCTCTAAGGACCTCATCAAGAAGCTGCTGGTGGTGGACAGG ACCCGACGCCTAGGCAACATGAAGAATGGGGCAGAAGACATCAAGCGGCACCGGTGGTTCCGAGGTGTGG AGTGGGAATCTGTGCCACAAAGAAAACTGAAGCCACCCATTGTGCCCAAGTTATCGGGTGATGGTGACAT CTCCAACTTTGAGACTTATCCAGAGAGTGAATTGGACAAGACACCTTCTGTATCTGACAAAGACCTGGAA ACATTCAAAAATTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205408 protein sequence
Red=Cloning site Green=Tags(s) MEPPAGAAATVKDPDHDPVKTKVSAPAADPKPRTSSQKAGHSLQDWDTIATVGTGTFGRVNLVKEKTGRQ YCALKIMSIPDVIRLKQEQHVQNEKAVLKEINHPFLIKLLWTGHDNRFLYMLMEFVPGGELFTYLRNRGR FSSVASVFYATEIVCAIEYLHSKEIVYRDLKPENILLDREGHIKLTDFGFAKKLVDRTWTLCGTPEYLAP EVIQSKGHGRAVDWWALGILIFEMLSGFPPFFDDNPFGIYQKILACKIDFPRQLDFTSKDLIKKLLVVDR TRRLGNMKNGAEDIKRHRWFRGVEWESVPQRKLKPPIVPKLSGDGDISNFETYPESELDKTPSVSDKDLE TFKNF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016979 |
| ORF Size | 1068 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016979.2 |
| RefSeq Size | 3217 bp |
| RefSeq ORF | 1068 bp |
| Locus ID | 19108 |
| UniProt ID | Q922R0 |
| MW | 40.5 kDa |
| Gene Summary | Serine/threonine protein kinase regulated by and mediating cAMP signaling in cells. Acts through phosphorylation of downstream targets that may include CREB, SMAD6 and PKD1 and has multiple functions in cellular differentiation and epithelial morphogenesis. Regulates myeloid cell differentiation through SMAD6 phosphorylation. Involved in nephrogenesis by stimulating renal epithelial cell migration and tubulogenesis. Also involved in angiogenesis through stimulation of endothelial cell proliferation, migration and vascular-like structure formation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC207359 | Prkx (untagged) - Mouse protein kinase, X-linked (Prkx), (10ug) |
CNY 3,990.00 |
|
| MG205408 | Prkx (tGFP-tagged) - Mouse protein kinase, X-linked (Prkx) |
CNY 3,140.00 |
|
| MR205408L3 | Lenti ORF clone of Prkx (Myc-DDK-tagged) - Mouse protein kinase, X-linked (Prkx) |
CNY 4,750.00 |
|
| MR205408L4 | Lenti ORF clone of Prkx (mGFP-tagged) - Mouse protein kinase, X-linked (Prkx) |
CNY 4,750.00 |
