United States
Germany
Japan
United Kingdom
China
Psmc5 (BC030840) Mouse Tagged ORF Clone
CAT＃: MR205252
- TrueORF®
Psmc5 (Myc-DDK-tagged) - Mouse protease (prosome, macropain) 26S subunit, ATPase 5 (cDNA clone MGC:31044 IMAGE:3994523)
ORF Plasmid: tGFP
"BC030840" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | mSUG1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR205252 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTTGATGGGCCAGAGCAGATGGAACTGGAAGAGGGGAAGGCAGGCAGTGGACTCCGTCAATATT ATCTGTCCAAGATTGAAGAACTCCAGTTGATTGTAAATGATAAGAGCCAGAATCTCCGTAGACTGCAGGC ACAGAGGAATGAGCTGAATGCAAAAGTTCGCCTGTTGCGGGAGGAGCTGCAGCTGTTGCAGGAACAGGGC TCCTACGTTGGAGAAGTCGTGAGGGCCATGGATAAGAAAAAAGTATTGGTCAAGGTCCATCCTGAGGGCA AATTTGTTGTTGATGTGGACAAGAACATTGATATCAACGATGTGACGCCCAATTGTCGGGTCGCTCTAAG AAATGACAGCTACACTCTGCATAAGATCTTACCTAACAAGGTGGACCCTTTGGTGTCACTAATGATGGTG GAGAAGGTGCCAGACTCAACCTACGAGATGATTGGCGGCCTGGACAAGCAGATCAAGGAGATTAAAGAAG TGATCGAGCTGCCCGTGAAGCACCCCGAGCTCTTTGAAGCACTGGGCATCGCACAGCCAAAGGGAGTCCT GCTCTACGGACCCCCAGGCACTGGGAAGACATTGTTGGCCCGCGCTGTGGCTCATCATACAGACTGTACC TTTATTCGTGTCTCTGGCTCTGAACTGGTACAGAAATTCATCGGGGAAGGGGCAAGAATGGTGAGGGAGC TGTTTGTCATGGCCCGAGAACATGCTCCATCCATCATCTTCATGGACGAGATTGACTCTATTGGCTCCTC ACGGCTGGAGGGGGGCTCTGGAGGCGACAGTGAGGTACAGCGCACGATGCTGGAACTGCTCAATCAGCTG GATGGCTTTGAGGCCACCAAGAATATCAAGGTTATCATGGCTACTAATAGGATTGATATCCTGGACTCTG CCCTGCTTCGTCCTGGGAGGATTGACAGAAAAATTGAATTCCCACCCCCCAACGAGGAGGTCTGTGCTGG AGGTGCCTGGAGAAGCTCTGGCTATGGAGACAGTGCAGGGCTTAGGGCTTTCTTCTCTTATCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205252 protein sequence
Red=Cloning site Green=Tags(s) MALDGPEQMELEEGKAGSGLRQYYLSKIEELQLIVNDKSQNLRRLQAQRNELNAKVRLLREELQLLQEQG SYVGEVVRAMDKKKVLVKVHPEGKFVVDVDKNIDINDVTPNCRVALRNDSYTLHKILPNKVDPLVSLMMV EKVPDSTYEMIGGLDKQIKEIKEVIELPVKHPELFEALGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCT FIRVSGSELVQKFIGEGARMVRELFVMAREHAPSIIFMDEIDSIGSSRLEGGSGGDSEVQRTMLELLNQL DGFEATKNIKVIMATNRIDILDSALLRPGRIDRKIEFPPPNEEVCAGGAWRSSGYGDSAGLRAFFSYP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC030840 |
| ORF Size | 1044 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC030840, AAH30840 |
| RefSeq Size | 1415 bp |
| RefSeq ORF | 1046 bp |
| Locus ID | 19184 |
| MW | 38.8 kDa |
| Gene Summary | Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC5 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217736 | Psmc5 (untagged) - Mouse protease (prosome, macropain) 26S subunit, ATPase 5 (cDNA clone MGC:31044 IMAGE:3994523), (10ug) |
CNY 6,940.00 |
|
| MG205252 | Psmc5 (tGFP-tagged) - Mouse protease (prosome, macropain) 26S subunit, ATPase 5 (cDNA clone MGC:31044 IMAGE:3994523) |
CNY 2,190.00 |
|
| MR205252L3 | Lenti ORF clone of Psmc5 (Myc-DDK-tagged) - Mouse protease (prosome, macropain) 26S subunit, ATPase 5 (cDNA clone MGC:31044 IMAGE:3994523) |
CNY 3,900.00 |
|
| MR205252L4 | Lenti ORF clone of Psmc5 (mGFP-tagged) - Mouse protease (prosome, macropain) 26S subunit, ATPase 5 (cDNA clone MGC:31044 IMAGE:3994523) |
CNY 3,900.00 |
