Klf17 (NM_029416) Mouse Tagged ORF Clone
CAT#: MR205129
- TrueORF®
Klf17 (Myc-DDK-tagged) - Mouse Kruppel-like factor 17 (Klf17)
ORF Plasmid: tGFP
"NM_029416" in other vectors (4)
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CNY 3,656.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 7420700M05Rik; AA420409; AU043468; C85123; D4Ertd561e; Gzf; Zfp393 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR205129 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCAGGACAATAAGGAACAGGCTATGCACCAGCCTCCCATGGATAATAAGATGTTGGTGCCAGTGT CGAATGTGCCCGTGTCTTCTGGAAACAGCGGATTTCATCAGCCACCAGCCACTCAATACTTACCAGAGAT GATGAGGTCCTATATGGCATCAGCTGAGGAGCTCAGATGCAATGAGAGAGAATGGGAATCACAGCTCATT AGGTCGCTGCCTGAGCATGGTGTGAGGTGCCCCTCCCAGCTGGCTCCTATACCTTTCCAGAATTATTGTC AGAGATCGATTGGGAGGGGATCCCACGTGATGCCAGTTGGAAGTTCAGGTACTCTGGGAGTGACCATTTC CTTCAGTGAGAATCTAATGCCTCAGGGTGGCCTGCCAAGTTCCCGTGGAGTCTCAGTGATGGCCCATAGC GGTGCCCCAGCAATGCCTTATCCTATGCCTCCAACAGTACCTGCCACCACGGGCTCTTTAAAACATGGAA TATTGTTGGTCCCAGGCATGGCTTCCGCTGGGACCCATGCTGTGGCTCCGTTTATGGATCAGATGTTGCA CTCCATAAATCCCTGCAATCCTGAGATGCTCCCAGCTAGGTTCCAGCAATTGCTACCTTTAGACTCCCAA GATTCACTTGTGACTGAGTCAAATACCCAGGAAGAACCTTTCGTACGTGAGCCGCCCACACCTGCTCCAG AGGGGGCAGAGAGCCCCAGTACTTCAAGAGGGGCAACCAGGAGACAGTCCCCAGTTTCAAGGCCTTATGT CTGCACATATAACAGCTGTGGAAAATCTTATACCAAGCGCTCTCACCTTGTGAGTCACCAACGCAAACAC ACAGGTGTGAAGCCCTTCGCATGTGACTGGAACGGATGTACGTGGAAGTTCTTCCGCTCTGATGAGCTTG GACGACACAAACGGATTCATACCAGATACCGACCACATAAATGCGATGAGTGTGATCGAGAGTTCATGAG ATCTGACCATCTCAGGCAGCACAAAAGAACGCATCTGCCCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205129 protein sequence
Red=Cloning site Green=Tags(s) MEQDNKEQAMHQPPMDNKMLVPVSNVPVSSGNSGFHQPPATQYLPEMMRSYMASAEELRCNEREWESQLI RSLPEHGVRCPSQLAPIPFQNYCQRSIGRGSHVMPVGSSGTLGVTISFSENLMPQGGLPSSRGVSVMAHS GAPAMPYPMPPTVPATTGSLKHGILLVPGMASAGTHAVAPFMDQMLHSINPCNPEMLPARFQQLLPLDSQ DSLVTESNTQEEPFVREPPTPAPEGAESPSTSRGATRRQSPVSRPYVCTYNSCGKSYTKRSHLVSHQRKH TGVKPFACDWNGCTWKFFRSDELGRHKRIHTRYRPHKCDECDREFMRSDHLRQHKRTHLPK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_029416 |
ORF Size | 1026 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_029416.1, NM_029416.2, NP_083692.2 |
RefSeq Size | 3595 bp |
RefSeq ORF | 1026 bp |
Locus ID | 75753 |
UniProt ID | Q8CFA7 |
MW | 38 kDa |
Gene Summary | Transcription repressor that binds to the promoter of target genes and prevents their expression. Acts as a negative regulator of epithelial-mesenchymal transition and metastasis in breast cancer. Specifically binds the 5'-CACCC-3' sequence in the promoter of ID1, a key metastasis regulator in breast cancer, and repress its expression. May be a germ cell-specific transcription factor that plays important roles in spermatid differentiation and oocyte development.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC204853 | Klf17 (untagged) - Mouse Kruppel-like factor 17 (Klf17), (10ug) |
CNY 3,656.00 |
|
MG205129 | Klf17 (tGFP-tagged) - Mouse Kruppel-like factor 17 (Klf17) |
CNY 3,140.00 |
|
MR205129L3 | Lenti ORF clone of Klf17 (Myc-DDK-tagged) - Mouse Kruppel-like factor 17 (Klf17) |
CNY 4,750.00 |
|
MR205129L4 | Lenti ORF clone of Klf17 (mGFP-tagged) - Mouse Kruppel-like factor 17 (Klf17) |
CNY 4,750.00 |