Srp68 (BC033573) Mouse Tagged ORF Clone
CAT#: MR204949
- TrueORF®
Srp68 (Myc-DDK-tagged) - Mouse signal recognition particle 68 (cDNA clone MGC:38208 IMAGE:5323201)
ORF Plasmid: tGFP
"BC033573" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MGC38208 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR204949 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGAGGTGGAGTGGCGAGGCCGGACGGTCCCCGTGAAGATCGACAAAGTGAGGATCTTCCTCCTGG GCCTGGCCGACAACGAGGCCGCCATCGTCCAGGCTGAAAGTGAAGAAACGAAGGAGCGTCTGTTCGAGTC CATGCTGAGTGAGTGCCGAGACGCCCTCCAAGCCGTGAGGGAAGAGCTCAAGCCCGATCAGAAGCAGAGA GACTATGCCCTTGATGGAGAGTCTGGGAAGGTGTCAAATCTCCAGTACCTGCACAGCTACCTGACCTACA TCAAGCTGTCAACAGCAATCAGGCGAAACGAGAACATGGCTAAAGGCTTGCACAGGGCTCTGCTGCAGCA GCAGCCGGAAGATGACAGCAAGCGCTCTCCCAGGCCTCAGGACCTCATCCGGCTCTATGACATCATTCTG CAGAATCTGGTGGAATTGCTGCAGCTTCCTGGCTTAGAAGAGGACAGAACCTTCCAGAAAGAGATAAGCC TCAAGACCCTGGTGTTCAAAGCTTACAGGTGTTTCTTCATTGCTCAGTCTTACGTGCTGGTGAAGAAGTG GAGCGAAGCCCTCGTCCTGTACGACAGAGTTCTCAAATATGCAAATGAAGTGAGTTCTCACGGAGGAGCC TCCAAGAACAGCCTGAAGGACCTGCCCGACGTGCAAGAACTCATCACTCAAGTGCGGTCAGAGAAGTGCT CGCTGCAGGCCGCAGCCATCCTGGATGCAAACGACTCTCATCAAACAGACACCTCATCCCAAGTCAAAGA CAATACGCCTCTGGTTGAACGGTTTGAGTCCTTTTGCCTGGACCCGTCCCTGGTCACCAAACAAGCCAAC CTCGTGCACTTCCCACCAGGGTTCCAGCCCATCCCTTGCAAGCCTTTGTTCTTTGACCTGGCCCTCAACC ACGTGGCCTTCCCACCCCTTGAGGACAAATTGGAGCAGAAGACTAAGAGTGGCCTCACAGGCTACATCAA GGGCATCTTTGGTTTCAGGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204949 protein sequence
Red=Cloning site Green=Tags(s) MSEVEWRGRTVPVKIDKVRIFLLGLADNEAAIVQAESEETKERLFESMLSECRDALQAVREELKPDQKQR DYALDGESGKVSNLQYLHSYLTYIKLSTAIRRNENMAKGLHRALLQQQPEDDSKRSPRPQDLIRLYDIIL QNLVELLQLPGLEEDRTFQKEISLKTLVFKAYRCFFIAQSYVLVKKWSEALVLYDRVLKYANEVSSHGGA SKNSLKDLPDVQELITQVRSEKCSLQAAAILDANDSHQTDTSSQVKDNTPLVERFESFCLDPSLVTKQAN LVHFPPGFQPIPCKPLFFDLALNHVAFPPLEDKLEQKTKSGLTGYIKGIFGFRS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | BC033573 |
ORF Size | 1002 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC033573, AAH33573 |
RefSeq Size | 1870 bp |
RefSeq ORF | 1004 bp |
Locus ID | 217337 |
MW | 38 kDa |
Gene Summary | Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP68 binds the 7S RNA, SRP72 binds to this complex subsequently. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
The Perlman syndrome DIS3L2 exoribonuclease safeguards endoplasmic reticulum-targeted mRNA translation and calcium ion homeostasis
,Pirouz, M;Wang, CH;Liu, Q;Ebrahimi, AG;Shamsi, F;Tseng, YH;Gregory, RI;,
Nat Commun
,PubMed ID 32457326
[SRP68]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG204949 | Srp68 (tGFP-tagged) - Mouse signal recognition particle 68 (cDNA clone MGC:38208 IMAGE:5323201) |
CNY 2,090.00 |
|
MR204949L3 | Lenti ORF clone of Srp68 (Myc-DDK-tagged) - Mouse signal recognition particle 68 (cDNA clone MGC:38208 IMAGE:5323201) |
CNY 3,800.00 |
|
MR204949L4 | Lenti ORF clone of Srp68 (mGFP-tagged) - Mouse signal recognition particle 68 (cDNA clone MGC:38208 IMAGE:5323201) |
CNY 3,800.00 |