Chrdl1 (NM_031258) Mouse Tagged ORF Clone
CAT#: MR204928
- TrueORF®
Chrdl1 (Myc-DDK-tagged) - Mouse chordin-like 1 (Chrdl1), transcript variant 2
ORF Plasmid: tGFP
"NM_031258" in other vectors (4)
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CNY 2,400.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CHL; CHL1; Nrln1; VOPT |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR204928 representing NM_031258
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGGCATGAAATACATCATTTCCTTATTTTTCATCTTTGTTTTCCTAGAAGGAAGCAAAACAGAAC AAGTAAAACACTCAGACACATATTGCGTGTTTCAAGACAAGAAGTATAGAGTGGGTGAGAAATGGCATCC CTACCTGGAACCGTATGGACTGGTTTACTGTGTGAACTGCATCTGCTCTGAGAATGGGAATGTGCTTTGC AGCCGAGTCAGATGTCCAAGTCTTCATTGCCTTTCACCCGTGCATATTCCTCATCTCTGTTGCCCCCGCT GCCCAGACTCCTTACCACCAGTGAACAATAAGGTGACCAGCAAGTCATGCGAATACAATGGAACCACTTA CCAACATGGAGAACTGTTCATAGCTGAAGGGCTCTTTCAGAACCGGCAACCCAATCAGTGCAGTCAGTGT AGCTGCTCGGAGGGGAATGTATACTGTGGTCTCAAGACTTGCCCCAAACTGACCTGTGCATTCCCAGTCT CTGTTCCAGATTCTTGCTGCCGAGTATGCAGAGGGGATGCAGAATTATCGTGGGAACATGCGGATGGTGA TATCTTCCGGCAACCTGCCAACAGAGAAGCAAGACATTCTTACCTCCGTTCCCCCTACGATCCTCCACCA AGCAGACAAGCTGGAGGTCTTCCCCGCTTTCCTGGGAGCAGAAGTCACCGGGGAGCTGTTATAGATTCCC AGCAAGCATCCGGGACCATCGTGCAGATTGTCATCAATAACAAGCACAAACATGGACAAGTGTGTGTTTC CAATGGAAAGACCTACTCTCATGGAGAGTCCTGGCACCCAAATCTACGAGCATTTGGCATTGTGGAATGT GTACTATGCACTTGTAATGTCACCAAGCAAGAATGTAAGAAAATCCACTGCCCCAATCGATACCCCTGCA AGTATCCTCAAAAAATAGATGGAAAGTGCTGCAGGGTGTGCCCAGGTAAAAAGGCAAAAGGTGCATTGGC TGGAGGCCCTGCCTTTGGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204928 representing NM_031258
Red=Cloning site Green=Tags(s) MDGMKYIISLFFIFVFLEGSKTEQVKHSDTYCVFQDKKYRVGEKWHPYLEPYGLVYCVNCICSENGNVLC SRVRCPSLHCLSPVHIPHLCCPRCPDSLPPVNNKVTSKSCEYNGTTYQHGELFIAEGLFQNRQPNQCSQC SCSEGNVYCGLKTCPKLTCAFPVSVPDSCCRVCRGDAELSWEHADGDIFRQPANREARHSYLRSPYDPPP SRQAGGLPRFPGSRSHRGAVIDSQQASGTIVQIVINNKHKHGQVCVSNGKTYSHGESWHPNLRAFGIVEC VLCTCNVTKQECKKIHCPNRYPCKYPQKIDGKCCRVCPGKKAKGALAGGPAFG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_031258 |
ORF Size | 999 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_031258.1, NM_031258.2, NM_031258.3, NP_112548.2 |
RefSeq Size | 3291 bp |
RefSeq ORF | 1002 bp |
Locus ID | 83453 |
UniProt ID | Q920C1 |
MW | 37.5 kDa |
Gene Summary | Seems to antagonize the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation (By similarity). Antagonizes the function of BMP7 and may thus play an important role in the embryonic bone formation. Shows no inhibitory effect on the inducing activity of BMP2. Plays a role during anterior segment eye development (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
Stage-Specific Modulation of Cortical Neuronal Development by Mmu-miR-134
,Philip Gaughwin, Maciej Ciesla, Henry Yang, Bing Lim, and Patrik Brundin,
Cereb Cortex, Jan 2011; 10.1093/cercor/bhq262
[Chrdl1]
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Documents
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FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC207786 | Chrdl1 (untagged) - Mouse chordin-like 1 (Chrdl1), transcript variant 2, (10ug) |
CNY 3,990.00 |
|
MG204928 | Chrdl1 (tGFP-tagged) - Mouse chordin-like 1 (Chrdl1) |
CNY 4,000.00 |
|
MR204928L3 | Lenti ORF clone of Chrdl1 (Myc-DDK-tagged) - Mouse chordin-like 1 (Chrdl1), transcript variant 2 |
CNY 4,750.00 |
|
MR204928L4 | Lenti ORF clone of Chrdl1 (mGFP-tagged) - Mouse chordin-like 1 (Chrdl1), transcript variant 2 |
CNY 4,750.00 |