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Ift74 (BC049156) Mouse Tagged ORF Clone
CAT＃: MR204909
- TrueORF®
Ift74 (Myc-DDK-tagged) - Mouse intraflagellar transport 74 homolog (Chlamydomonas) (cDNA clone MGC:56922 IMAGE:6313441)
ORF Plasmid: tGFP
"BC049156" in other vectors (4)
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CNY 3,330.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1700029H06Rik; b2b796Clo; Ccdc2; Cmg1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR204909 representing BC049156
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGTAATCACAAATCTTCAGCCCCTCGCCCTATTTCTCGGGGTGGAATAGGACTAACAGGAAGGC CTCCTTCTGGAATAAGACCTCCATCTGGCAATGTTCGAGTGGCAACTGCAATGCCACCAACAACAGCAAG ACCAGGTTCTCGTGGTGGTCCCTTAGGGACTGGTGGAGTTTTGTCATCTCAAATCAAAGTTGCTGATCGT CCTGTGACCCAACAAGGTTTGAGTGGAATGAAGACTGGCATGAAAGGTCCCCAGAGGCAAATTTTAGACA AATCTTACTATCTTGGACTTCTTAGGAGCAAAATAAGTGAACTTACAACTGAAATTAATAAGCTTCAGAA AGAAATAGAAATGTACAACCAAGAAAATTCAGTGTATTTGTCATATGAAAAGAGGGCAGAGACATTAGCT GTTGAAATTAAAGACTTTCAAGGACAACTAGCAGACTACAACATGTTGGTAGATAAACTTAATACCAATA CTGAAATGGAAGAAGTAATGAGTGATTACAACATGCTTAAAGCTCAAAATGACAGAGAAACACAAAGTAT GGATGTCATATTTACTGAAAGACAAGCGAAGGAAAAACAAATCAGAAGTGTAGAAGAAGAAGTTGAACAA GAAAAGCAAGCAGCAGATGGCATTATCAAAAATATGTCTCCTGAAAAACAAGTCAAGTACATAGAAATGA AAACCACAAATGAGAAACTGTTGCAGGAATTAGATACACTTCAGCAACAGCTAGATTCTCTGAACATGAA AAAGGAGAGCCTAGAAACTGAAATAGCACACTCCCAAGTAAAACAAGAGGCTGTATTGCTGTATGAAAAA CTTTATGAGTTAGAGTCCCATCGAGATCAAATGATTGCAGAAGACAAAAGCATGGGATCGCCAATGGAGG AAAGGGAGAGATTGTTTAAGCAGGTTAAAGAAGATAATCAGGAAATAGCCAGCATGGAGAGACAGTTAAC AGATATAAAAGAAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204909 representing BC049156
Red=Cloning site Green=Tags(s) MASNHKSSAPRPISRGGIGLTGRPPSGIRPPSGNVRVATAMPPTTARPGSRGGPLGTGGVLSSQIKVADR PVTQQGLSGMKTGMKGPQRQILDKSYYLGLLRSKISELTTEINKLQKEIEMYNQENSVYLSYEKRAETLA VEIKDFQGQLADYNMLVDKLNTNTEMEEVMSDYNMLKAQNDRETQSMDVIFTERQAKEKQIRSVEEEVEQ EKQAADGIIKNMSPEKQVKYIEMKTTNEKLLQELDTLQQQLDSLNMKKESLETEIAHSQVKQEAVLLYEK LYELESHRDQMIAEDKSMGSPMEERERLFKQVKEDNQEIASMERQLTDIKEK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC049156 |
| ORF Size | 996 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC049156 |
| RefSeq Size | 1659 bp |
| RefSeq ORF | 998 bp |
| Locus ID | 67694 |
| MW | 60.8 kDa |
| Gene Summary | Component of the intraflagellar transport (IFT) complex B: together with IFT81, forms a tubulin-binding module that specifically mediates transport of tubulin within the cilium. Binds beta-tubulin via its basic region. Required for ciliogenesis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218386 | Ift74 (untagged) - Mouse intraflagellar transport 74 homolog (Chlamydomonas) (cDNA clone MGC:56922 IMAGE:6313441), (10ug) |
CNY 6,940.00 |
|
| MG204909 | Ift74 (tGFP-tagged) - Mouse intraflagellar transport 74 homolog (Chlamydomonas) (cDNA clone MGC:56922 IMAGE:6313441) |
CNY 3,710.00 |
|
| MR204909L3 | Lenti ORF clone of Ift74 (Myc-DDK-tagged) - Mouse intraflagellar transport 74 homolog (Chlamydomonas) (cDNA clone MGC:56922 IMAGE:6313441) |
CNY 5,230.00 |
|
| MR204909L4 | Lenti ORF clone of Ift74 (mGFP-tagged) - Mouse intraflagellar transport 74 homolog (Chlamydomonas) (cDNA clone MGC:56922 IMAGE:6313441) |
CNY 5,230.00 |
