Dnttip1 (NM_133763) Mouse Tagged ORF Clone
CAT#: MR204812
- TrueORF®
Dnttip1 (Myc-DDK-tagged) - Mouse deoxynucleotidyltransferase, terminal, interacting protein 1 (Dnttip1)
ORF Plasmid: tGFP
"NM_133763" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 6430706C13Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR204812 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGCGACTGGCGACACCGAGCAGCCGCGGGGCCCCGGCGGGGCGGAGCGAGGTGGCCTGGAGCTGG GCGACGCGGGCGCGGCGGGCCAGCCGGTTCTCACGAACCCTTGGAACATAATGATAAAACATCGGCAGGT GCAGCGAAGGGGCCGCCGATCTCAGATGACCACAAGTTTCACAGACCCAGCCATCTCTATGGATCTCCTC CGTGCTGTCCTGCAGCCTAGCATCAATGAGGAGATCCAGGGTGTCTTCAACAAGTACATGAAGTTCTTCC AGAAGGCAGCGCTGAATGTGCGAGACAATGTTGGGGAAGAAGTGGACGCAGAGCAGTTGATTCAGGAGGC CTGCCGCAGCTGCCTGGAGCAGGCAAAGCTGCTCTTTTCTGATGGAGAGAAAGTGATACCCAGATTGGCC CATGAGCTTCCAGGGATCAAGCGGGGCCGGCAAGCAGAAGAGGAGTCCCACCGAGGAAGCCCCATTCCCA AAAAGAGGAAAGGTCGGCCTCCTGGACACGTCCTGTCAAATGACCGCGCAGCTGCTGGCATGGTATGGAA ACAAAAATCCTGTGAACCAATTCGCCGAGAAGGCCCCAAGTGGGACCCAGCTCGGCTGAATGAATCTACC ACCTTTGTTTTGGGGTCTCGAGCCAACAAGGCCTTGGGGATGGGAGGCACCAGAGGGAGAATCTACATCA AGCACCCACACCTCTTTAAGTATGCAGCAGATCCTCAGGACAAGCACTGGCTGGCTGAGCAGCATCACAT GCGGGCAACAGGCGGAAAGATGGCGTACCTTCTCATTGAAGAAGACATCCGGGACTTGGCTGCCAGCGAT GACTACAGAGGATGCTTGGACCTGAAGTTGGAGGAGCTGAAATCCTTTGTTCTGCCATCCTGGATGGTTG AGAAGATGCGGAAATACATGGAGACACTGCGGACAGAAAATGAGCACCGCGCTGCGGAAGCGCCTCCCCA GACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204812 protein sequence
Red=Cloning site Green=Tags(s) MGATGDTEQPRGPGGAERGGLELGDAGAAGQPVLTNPWNIMIKHRQVQRRGRRSQMTTSFTDPAISMDLL RAVLQPSINEEIQGVFNKYMKFFQKAALNVRDNVGEEVDAEQLIQEACRSCLEQAKLLFSDGEKVIPRLA HELPGIKRGRQAEEESHRGSPIPKKRKGRPPGHVLSNDRAAAGMVWKQKSCEPIRREGPKWDPARLNEST TFVLGSRANKALGMGGTRGRIYIKHPHLFKYAADPQDKHWLAEQHHMRATGGKMAYLLIEEDIRDLAASD DYRGCLDLKLEELKSFVLPSWMVEKMRKYMETLRTENEHRAAEAPPQT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_133763 |
ORF Size | 987 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_133763.1, NP_598524.1 |
RefSeq Size | 1257 bp |
RefSeq ORF | 987 bp |
Locus ID | 76233 |
UniProt ID | Q99LB0 |
MW | 36.9 kDa |
Gene Summary | Increases DNTT terminal deoxynucleotidyltransferase activity (in vitro). Also acts as a transcriptional regulator, binding to the consensus sequence 5'-GNTGCATG-3' following an AT-tract. Associates with RAB20 promoter and positively regulates its transcription. Binds DNA and nucleosomes; may recruit HDAC1 complexes to nucleosomes or naked DNA.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200447 | Dnttip1 (untagged) - Mouse deoxynucleotidyltransferase, terminal, interacting protein 1 (Dnttip1), (10ug) |
CNY 2,400.00 |
|
MG204812 | Dnttip1 (tGFP-tagged) - Mouse deoxynucleotidyltransferase, terminal, interacting protein 1 (Dnttip1) |
CNY 2,850.00 |
|
MR204812L3 | Lenti ORF clone of Dnttip1 (Myc-DDK-tagged) - Mouse deoxynucleotidyltransferase, terminal, interacting protein 1 (Dnttip1) |
CNY 4,750.00 |
|
MR204812L4 | Lenti ORF clone of Dnttip1 (mGFP-tagged) - Mouse deoxynucleotidyltransferase, terminal, interacting protein 1 (Dnttip1) |
CNY 4,750.00 |
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