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Trit1 (BC019812) Mouse Tagged ORF Clone
CAT＃: MR204764
- TrueORF®
Trit1 (Myc-DDK-tagged) - Mouse tRNA isopentenyltransferase 1 (cDNA clone MGC:30541 IMAGE:5042856)
ORF Plasmid: tGFP
"BC019812" in other vectors (4)
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CNY 3,230.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | IPT, MOD5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR204764 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCACTGGGAAAGTGGTTGATCGGAAAGTAGAGCTTGAAAAGGAAGATGGTCATGAACTCCATAAAC GGCTAAGCCAGGTGGACCCAGAAATGGCCGCCAAGCTACACCCGCATGACAAACGCAAAGTGGCCAGGAG CTTGCAAGTGTTTGAAGAAACAGGAATCTCTCACAGTGAATTCCTTCATCGTCAGCACGCAGAGGAAGGC GGTGGTCCTCTTGGAGGCCCTCTCAGGTTCCCTAACCCATGCATCCTCTGGCTCCATGCTGACCAGGCAG TTCTAGATGAGCGCTTGGATAAAAGAGTGGATGACATGCTTGCTGCCGGACTCTTGGAAGAGCTGAGAGG TTTTCACAGACGTTATAATCTTAAGAACATTTCAGAAAATAGCCAGGACTATCAACACGGTATCTTCCAG TCAATTGGCTTCAAGGAATTTCACGAGTACCTGACCACTGAGGGGAAATGCACACCAGAGACTAGTAACC AGCTTCTAAAGAAAGGTATTGAGGCTCTGAAACAAGTAACTAAGAGATATGCCCGGAAACAGAACCGATG GGTTAAAAACCGCTTTTTGAGCAGACCTGGGCCCAGTGTCCCCCCGGTATATGGCTTAGAAGTATCTGAT GTTTCCAAGTGGGAGGAGTCTGTTCTGGAACCTGCTCTCAATATAGTACAAAGTTTCATCCAGGGTCACA AACCTACAGCCATGCCAGTGAAGATGGCATACAATGAAAGTGAGAACAAGAGAAGTTACCACATGTGTGA CCTCTGTGACCGGATCATCATTGGGGACCGGGAATGGGCAGCACATTTAAAATCCAAATCTCACTTGCAC CAATTGAAGAAAAGAAGAAGGTTGGACTTAGACGCTGTCAGTGCCACAGGAAGTCAAAGTAATTCCCCAG ACTGTGACCCGGAACGCATCGAGGGGGAATCCTCGGGGCAGCACAATCAAGAGCTGAAGGCCAGTGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204764 protein sequence
Red=Cloning site Green=Tags(s) MGTGKVVDRKVELEKEDGHELHKRLSQVDPEMAAKLHPHDKRKVARSLQVFEETGISHSEFLHRQHAEEG GGPLGGPLRFPNPCILWLHADQAVLDERLDKRVDDMLAAGLLEELRGFHRRYNLKNISENSQDYQHGIFQ SIGFKEFHEYLTTEGKCTPETSNQLLKKGIEALKQVTKRYARKQNRWVKNRFLSRPGPSVPPVYGLEVSD VSKWEESVLEPALNIVQSFIQGHKPTAMPVKMAYNESENKRSYHMCDLCDRIIIGDREWAAHLKSKSHLH QLKKRRRLDLDAVSATGSQSNSPDCDPERIEGESSGQHNQELKASV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC019812 |
| ORF Size | 978 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC019812, AAH19812 |
| RefSeq Size | 2045 bp |
| RefSeq ORF | 980 bp |
| Locus ID | 66966 |
| MW | 37.2 kDa |
| Gene Summary | Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 of both cytosolic and mitochondrial tRNAs, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218374 | Trit1 (untagged) - Mouse tRNA isopentenyltransferase 1 (cDNA clone MGC:30541 IMAGE:5042856), (10ug) |
CNY 6,940.00 |
|
| MG204764 | Trit1 (tGFP-tagged) - Mouse tRNA isopentenyltransferase 1 (cDNA clone MGC:30541 IMAGE:5042856) |
CNY 3,520.00 |
|
| MR204764L3 | Lenti ORF clone of Trit1 (Myc-DDK-tagged) - Mouse tRNA isopentenyltransferase 1 (cDNA clone MGC:30541 IMAGE:5042856) |
CNY 5,130.00 |
|
| MR204764L4 | Lenti ORF clone of Trit1 (mGFP-tagged) - Mouse tRNA isopentenyltransferase 1 (cDNA clone MGC:30541 IMAGE:5042856) |
CNY 5,130.00 |
