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B3galt6 (BC082998) Mouse Tagged ORF Clone
CAT＃: MR204731
- TrueORF®
B3galt6 (Myc-DDK-tagged) - Mouse UDP-Gal:betaGal beta 1,3-galactosyltransferase, polypeptide 6 (cDNA clone MGC:91010 IMAGE:5011578), complete
ORF Plasmid: tGFP
"BC082998" in other vectors (3)
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CNY 2,400.00
CNY 6,365.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BB129894; GalTII |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR204731 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGTATTCCGGCGCGCTTGGCGGCACCGGGTGGCGCTGGGCCTAGGCGGCCTGGCGTTCTGCGGCA CCACTCTGTTGTACCTGGCGCGCTGCGCTTCCGAGGGCGAGACGCCCTCCGCTTCCGGAGCCGCTCGGCC CCGCGCTAAGGCCTTCCTGGCGGTGCTGGTGGCCAGTGCGCCCCGCGCGGTCGAGCGCCGCACCGCAGTG CGCAGCACGTGGCTGGCACCGGAGAGGCGTGGCGGACCCGAGGACGTGTGGGCGCGCTTCGCCGTGGGCA CTGGCGGCTTAGGCTCGGAGGAGCGGCGCGCTCTTGAGCTCGAGCAGGCGCAGCACGGGGACCTACTGCT GCTGCCCGCCCTGCGCGACGCCTACGAGAACCTCACGGCCAAGGTCCTGGCCATGCTGACCTGGCTGGAT GAGCGCGTGGACTTCGAGTTCGTGCTCAAGGCGGACGACGACTCCTTTGCGCGCCTGGACGCTATCCTGG TGGACCTACGCGCACGGGAGCCCGCACGCCGCCGGCGCCTCTACTGGGGCTTCTTTTCCGGGCGCGGGCG CGTCAAGCCGGGAGGTCGCTGGCGAGAAGCAGCCTGGCAACTCTGCGACTACTACCTGCCCTACGCGCTG GGCGGTGGCTATGTCCTTTCTGCGGACCTGGTGCATTACCTGCGCCTCAGCCGCGAATACCTGCGCGCGT GGCACAGTGAAGACGTATCGCTGGGCACCTGGCTGGCACCAGTGGATGTGCAACGGGAGCATGACCCACG CTTCGACACGGAGTACAAATCTCGAGGCTGCAACAATCAGTATCTGGTGACACACAAGCAAAGCCCAGAG GACATGTTGGAGAAGCAACAGATGTTGCTGCATGAGGGCCGGTTGTGCAAGCATGAGGTGCAGTTGCGCC TTTCCTATGTCTATGACTGGTCAGCTCCACCCTCCCAGTGCTGCCAGCGCAAGGAGGGCGTTCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204731 protein sequence
Red=Cloning site Green=Tags(s) MKVFRRAWRHRVALGLGGLAFCGTTLLYLARCASEGETPSASGAARPRAKAFLAVLVASAPRAVERRTAV RSTWLAPERRGGPEDVWARFAVGTGGLGSEERRALELEQAQHGDLLLLPALRDAYENLTAKVLAMLTWLD ERVDFEFVLKADDDSFARLDAILVDLRAREPARRRRLYWGFFSGRGRVKPGGRWREAAWQLCDYYLPYAL GGGYVLSADLVHYLRLSREYLRAWHSEDVSLGTWLAPVDVQREHDPRFDTEYKSRGCNNQYLVTHKQSPE DMLEKQQMLLHEGRLCKHEVQLRLSYVYDWSAPPSQCCQRKEGVP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC082998 |
| ORF Size | 975 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC082998, AAH82998 |
| RefSeq Size | 1599 bp |
| RefSeq ORF | 977 bp |
| Locus ID | 117592 |
| MW | 37 kDa |
| Gene Summary | Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal beta-linked galactose residue. Has a preference for galactose-beta-1,4-xylose that is found in the linker region of glycosaminoglycans, such as heparan sulfate and chondroitin sulfate. Has no activity towards substrates with terminal glucosamine or galactosamine residues (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Visualizing intra-Golgi localization and transport by side-averaging Golgi ministacks
,null,
The Journal of Cell Biology
,PubMed ID 35467701
[B3galt6]
|
|
The spatial separation of processing and transport functions to the interior and periphery of the Golgi stack
,Tie, HC;Ludwig, A;Sandin, S;Lu, L;,
Elife
,PubMed ID 30499774
[B3GALT6]
|
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG204731 | B3galt6 (tGFP-tagged) - Mouse UDP-Gal:betaGal beta 1,3-galactosyltransferase, polypeptide 6 (cDNA clone MGC:91010 IMAGE:5011578), complete |
CNY 2,850.00 |
|
| MR204731L3 | Lenti ORF clone of B3galt6 (Myc-DDK-tagged) - Mouse UDP-Gal:betaGal beta 1,3-galactosyltransferase, polypeptide 6 (cDNA clone MGC:91010 IMAGE:5011578), complete |
CNY 4,750.00 |
|
| MR204731L4 | Lenti ORF clone of B3galt6 (mGFP-tagged) - Mouse UDP-Gal:betaGal beta 1,3-galactosyltransferase, polypeptide 6 (cDNA clone MGC:91010 IMAGE:5011578), complete |
CNY 4,750.00 |
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