B3galt6 (BC082998) Mouse Tagged ORF Clone

CAT#: MR204731

  • TrueORF®

B3galt6 (Myc-DDK-tagged) - Mouse UDP-Gal:betaGal beta 1,3-galactosyltransferase, polypeptide 6 (cDNA clone MGC:91010 IMAGE:5011578), complete

ORF Plasmid: DDK tGFP



  "BC082998" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 2,400.00

CNY 6,365.00


货期*
现货

规格
    • 10 ug

Cited in 2 publications.

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms BB129894; GalTII
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR204731 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGTATTCCGGCGCGCTTGGCGGCACCGGGTGGCGCTGGGCCTAGGCGGCCTGGCGTTCTGCGGCA
CCACTCTGTTGTACCTGGCGCGCTGCGCTTCCGAGGGCGAGACGCCCTCCGCTTCCGGAGCCGCTCGGCC
CCGCGCTAAGGCCTTCCTGGCGGTGCTGGTGGCCAGTGCGCCCCGCGCGGTCGAGCGCCGCACCGCAGTG
CGCAGCACGTGGCTGGCACCGGAGAGGCGTGGCGGACCCGAGGACGTGTGGGCGCGCTTCGCCGTGGGCA
CTGGCGGCTTAGGCTCGGAGGAGCGGCGCGCTCTTGAGCTCGAGCAGGCGCAGCACGGGGACCTACTGCT
GCTGCCCGCCCTGCGCGACGCCTACGAGAACCTCACGGCCAAGGTCCTGGCCATGCTGACCTGGCTGGAT
GAGCGCGTGGACTTCGAGTTCGTGCTCAAGGCGGACGACGACTCCTTTGCGCGCCTGGACGCTATCCTGG
TGGACCTACGCGCACGGGAGCCCGCACGCCGCCGGCGCCTCTACTGGGGCTTCTTTTCCGGGCGCGGGCG
CGTCAAGCCGGGAGGTCGCTGGCGAGAAGCAGCCTGGCAACTCTGCGACTACTACCTGCCCTACGCGCTG
GGCGGTGGCTATGTCCTTTCTGCGGACCTGGTGCATTACCTGCGCCTCAGCCGCGAATACCTGCGCGCGT
GGCACAGTGAAGACGTATCGCTGGGCACCTGGCTGGCACCAGTGGATGTGCAACGGGAGCATGACCCACG
CTTCGACACGGAGTACAAATCTCGAGGCTGCAACAATCAGTATCTGGTGACACACAAGCAAAGCCCAGAG
GACATGTTGGAGAAGCAACAGATGTTGCTGCATGAGGGCCGGTTGTGCAAGCATGAGGTGCAGTTGCGCC
TTTCCTATGTCTATGACTGGTCAGCTCCACCCTCCCAGTGCTGCCAGCGCAAGGAGGGCGTTCCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR204731 protein sequence
Red=Cloning site Green=Tags(s)

MKVFRRAWRHRVALGLGGLAFCGTTLLYLARCASEGETPSASGAARPRAKAFLAVLVASAPRAVERRTAV
RSTWLAPERRGGPEDVWARFAVGTGGLGSEERRALELEQAQHGDLLLLPALRDAYENLTAKVLAMLTWLD
ERVDFEFVLKADDDSFARLDAILVDLRAREPARRRRLYWGFFSGRGRVKPGGRWREAAWQLCDYYLPYAL
GGGYVLSADLVHYLRLSREYLRAWHSEDVSLGTWLAPVDVQREHDPRFDTEYKSRGCNNQYLVTHKQSPE
DMLEKQQMLLHEGRLCKHEVQLRLSYVYDWSAPPSQCCQRKEGVP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC082998
ORF Size 975 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC082998, AAH82998
RefSeq Size 1599 bp
RefSeq ORF 977 bp
Locus ID 117592
MW 37 kDa
Gene Summary Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal beta-linked galactose residue. Has a preference for galactose-beta-1,4-xylose that is found in the linker region of glycosaminoglycans, such as heparan sulfate and chondroitin sulfate. Has no activity towards substrates with terminal glucosamine or galactosamine residues (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (2)

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