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Dpf3 (BC048572) Mouse Tagged ORF Clone
CAT＃: MR204514
- TrueORF®
Dpf3 (Myc-DDK-tagged) - Mouse D4, zinc and double PHD fingers, family 3 (cDNA clone MGC:58591 IMAGE:6705809)
ORF Plasmid: tGFP
"BC048572" in other vectors (4)
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CNY 3,140.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CERD4, cer-d4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR204514 representing BC048572
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTGCTTGCCCAAGGGCCACAACAGGCCTGGGGCCTGGATGGAGAAGAGGCACCGCGGCCCAGGCC TCGCTCCGGGCCAGTTGTACACATACCCTGCCCGCTGCTGGCGCAAGAAGCGACGATTGCACCCACCAGA GGACCCAAAACTACGACTCCTGGAAATCAAACCCGAAGTAGAACTGCCCCTGAAGAAAGATGGATTTACC TCTGAGAGTACCACACTGGAAGCCTTGCTTCGCGGCGAGGGAGTAGAGAAGAAGGTGGATGCCAGAGAAG AGGAAAGCATCCAGGAGATACAGAGGGTTTTGGAAAATGATGAAAACGTAGAAGAAGGGAATGAAGAGGA GGATTTGGAAGAAGATGTTCCCAAGCGCAAGAACAGGACCAGAGGACGGGCTCGCGGCTCTGCAGGCGGA AGGAGGAGGCATGATGCCGCCTCTCAGGAAGACCACGACAAACCCTACGTCTGCGACATCTGTGGCAAGC GCTACAAGAACCGGCCAGGACTCAGCTACCACTACGCTCATACTCACCTGGCCAGCGAGGAGGGAGACGA AGCCCAAGACCAGGAGACCCGATCCCCACCCAACCACAGAAATGAGAACCACAGACCCCAGAAAGGACCA GACGGGACAGTCATTCCTAATAACTACTGTGACTTCTGCTTGGGGGGCTCCAACATGAACAAGAAGAGTG GGAGGCCTGAAGAGCTGGTGTCCTGTGCAGACTGTGGACGCTCTGCTCATTTGGGAGGAGAAGGCAGGAA GGAGAAGGAGGCAGCGGCCGCAGCACGTACCACGGAGGACTTATTCGGTTCCACGTCAGAAAGTGACACC TCAACTTTCTACGGCTTTGATGAGGACGATTTGGAAGAGCCTCGCTCCTGTCGAGGACGCCGCAGTGGCC GGGGTTCACCCACAGCAGATAAAAAGGGCAGCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204514 representing BC048572
Red=Cloning site Green=Tags(s) MGCLPKGHNRPGAWMEKRHRGPGLAPGQLYTYPARCWRKKRRLHPPEDPKLRLLEIKPEVELPLKKDGFT SESTTLEALLRGEGVEKKVDAREEESIQEIQRVLENDENVEEGNEEEDLEEDVPKRKNRTRGRARGSAGG RRRHDAASQEDHDKPYVCDICGKRYKNRPGLSYHYAHTHLASEEGDEAQDQETRSPPNHRNENHRPQKGP DGTVIPNNYCDFCLGGSNMNKKSGRPEELVSCADCGRSAHLGGEGRKEKEAAAAARTTEDLFGSTSESDT STFYGFDEDDLEEPRSCRGRRSGRGSPTADKKGSC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC048572 |
| ORF Size | 945 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC048572.1 |
| RefSeq Size | 1123 bp |
| RefSeq ORF | 947 bp |
| Locus ID | 70127 |
| MW | 41.1 kDa |
| Gene Summary | Muscle-specific component of the BAF complex, a multiprotein complex involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Specifically binds acetylated lysines on histone 3 and 4 (H3K14ac, H3K9ac, H4K5ac, H4K8ac, H4K12ac, H4K16ac). In the complex, it acts as a tissue-specific anchor between histone acetylations and methylations and chromatin remodeling. It thereby probably plays an essential role in heart and skeletal muscle development (By similarity). Belongs to the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218412 | Dpf3 (untagged) - Mouse D4, zinc and double PHD fingers, family 3 (cDNA clone MGC:58591 IMAGE:6705809), (10ug) |
CNY 6,940.00 |
|
| MG204514 | Dpf3 (tGFP-tagged) - Mouse D4, zinc and double PHD fingers, family 3 (cDNA clone MGC:58591 IMAGE:6705809) |
CNY 3,420.00 |
|
| MR204514L3 | Lenti ORF clone of Dpf3 (Myc-DDK-tagged) - Mouse D4, zinc and double PHD fingers, family 3 (cDNA clone MGC:58591 IMAGE:6705809) |
CNY 5,040.00 |
|
| MR204514L4 | Lenti ORF clone of Dpf3 (mGFP-tagged) - Mouse D4, zinc and double PHD fingers, family 3 (cDNA clone MGC:58591 IMAGE:6705809) |
CNY 5,040.00 |
