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Ciapin1 (NM_134141) Mouse Tagged ORF Clone
CAT＃: MR204371
- TrueORF®
Ciapin1 (Myc-DDK-tagged) - Mouse cytokine induced apoptosis inhibitor 1 (Ciapin1)
ORF Plasmid: tGFP
"NM_134141" in other vectors (4)
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CNY 2,400.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2810413N20Rik; AA617265; anamorsin; AU021794 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR204371 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGTTTGGGATCTCCCCTGGCCAGCTTGTGGCAGTGTTCTGGGACAAGTCATCTCCTGAGGAAG CCCTAAAAAAGCTGGTGGCTAGACTGCAAGAGTTAACTGGCAGCGAGGGCCAGGTATTCATGGAAAACGT CACCCAGCTGTTGCAGTCTTCGCATAAGGAATCCAGCTTCGATGTCATTCTGTCGGGTGTAGTCCCAGGA AGCACCTCTCTGCACAGTGCTGAGGTTCTGGCTGAGATGGCCCGGATCCTCCGGCCAGGGGGCTGTCTTT TTCTGAAAGAACCAGTGGAGACAGCTGAAGTTAACAATGACAAAATGAAGACGGCCTCTAAGCTATGTTC AGCCCTGACTCTTTCTGGCCTCGTGGAAATAAAAGAGTTGCAGAGGGAGGCCTTAAGCCCTGAGGAGGTA CAGTCCGTGCAGGAGCACCTGGGCTACCACAGTGACAGCCTGCGCTCAGTCCGTGTCACTGGCAAGAAGC CAAACTTTGAAGTGGGTTCTTCTAGCCAGCTAAAGCTTCCCAACAAGAAGTCTTCTTCAGTGAAGCCTGT TGTGGATCCTGCTGCTGCCAAGCTCTGGACCCTCTCAGCAAATGACATGGAGGATGACAGTGTGGATCTC ATTGACTCAGACGAGCTGCTTGATCCAGAGGATTTGAAGAGGCCTGACCCAGCCTCCCTGAAGGCTCCTT CATGTGGGGAAGGGAAAAAGAGGAAGGCCTGTAAGAACTGCACCTGTGGCCTCGCAGAGGAACTGGAGCG GGAGCAGTCCAAGGCGCAGAGCTCTCAGCCCAAGTCAGCCTGTGGAAATTGCTACCTGGGTGACGCTTTC CGCTGTGCCAACTGCCCCTACCTCGGGATGCCAGCCTTCAAGCCTGGCGAGCAGGTGCTCCTGAGCAATA GCAATCTCCAGGATGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204371 protein sequence
Red=Cloning site Green=Tags(s) MEEFGISPGQLVAVFWDKSSPEEALKKLVARLQELTGSEGQVFMENVTQLLQSSHKESSFDVILSGVVPG STSLHSAEVLAEMARILRPGGCLFLKEPVETAEVNNDKMKTASKLCSALTLSGLVEIKELQREALSPEEV QSVQEHLGYHSDSLRSVRVTGKKPNFEVGSSSQLKLPNKKSSSVKPVVDPAAAKLWTLSANDMEDDSVDL IDSDELLDPEDLKRPDPASLKAPSCGEGKKRKACKNCTCGLAEELEREQSKAQSSQPKSACGNCYLGDAF RCANCPYLGMPAFKPGEQVLLSNSNLQDA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_134141 |
| ORF Size | 930 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_134141.4, NP_598902.1 |
| RefSeq Size | 4327 bp |
| RefSeq ORF | 930 bp |
| Locus ID | 109006 |
| UniProt ID | Q8WTY4 |
| MW | 33.4 kDa |
| Gene Summary | Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis, facilitating the de novo assembly of a [4Fe-4S] cluster on the scaffold complex NUBP1-NUBP2. Electrons are transferred to CIAPIN1 from NADPH via the FAD- and FMN-containing protein NDOR1. NDOR1-CIAPIN1 are also required for the assembly of the diferric tyrosyl radical cofactor of ribonucleotide reductase (RNR), probably by providing electrons for reduction during radical cofactor maturation in the catalytic small subunit (By similarity). Has anti-apoptotic effects in the cell. Involved in negative control of cell death upon cytokine withdrawal. Promotes development of hematopoietic cells (PubMed:14970183).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204059 | Ciapin1 (untagged) - Mouse cytokine induced apoptosis inhibitor 1 (Ciapin1), (10ug) |
CNY 2,400.00 |
|
| MG204371 | Ciapin1 (tGFP-tagged) - Mouse cytokine induced apoptosis inhibitor 1 (Ciapin1) |
CNY 2,850.00 |
|
| MR204371L3 | Lenti ORF clone of Ciapin1 (Myc-DDK-tagged) - Mouse cytokine induced apoptosis inhibitor 1 (Ciapin1) |
CNY 4,750.00 |
|
| MR204371L4 | Lenti ORF clone of Ciapin1 (mGFP-tagged) - Mouse cytokine induced apoptosis inhibitor 1 (Ciapin1) |
CNY 4,750.00 |
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