United States
Germany
Japan
United Kingdom
China
Ankmy2 (BC012275) Mouse Tagged ORF Clone
CAT＃: MR204185
- TrueORF®
Ankmy2 (Myc-DDK-tagged) - Mouse ankyrin repeat and MYND domain containing 2 (cDNA clone MGC:18754 IMAGE:4010781)
ORF Plasmid: tGFP
"BC012275" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
CNY 6,365.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ZMYND20, MGC18754 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR204185 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTCACATAAAGAAAGGCGAGCTGACCCAGGAGGAAAAGGAACTGCTGGAAGTGATCGGGAAAGGTA CTGTCCAAGAAGCCGGAAGACTGTTGTCTAGTAAGAATGTTCATGTCAACTGCTTGGATGAGAATGGAAT GACTCCTTTGATGCATGCAGCCTATAAAGGGAAACTCGAGATGTGCAAGCTCCTCCTGCGCCATGGAGCT GACGCCAGTTGCCATCAGCATGAGCACGGGTACACAGCCCTCATGTTTGCTGCGCTTTCCGGTAATAAAG ACATCACTTGGGTTATGCTGGAAGCAGGCGCAGAGACCGATGTTGTGAACTCGGTGGGAAGAACTGCTGC CCAGATGGCAGCCTTTGTGGGTCAGCACGATTGCGTGGCTATCATCAACAATTTCTTCCCTCGAGAGAGA CTGGATTATTACACTAAACCCCAGGGACTGGATAAAGAGCCAAAACTGCCTCCAAAGTTGGCAGGCCCGC TACACAAAATCATCACCACAACGAATCTCCACCCTGTCAAGATCGTGATGCTTGTGAGTGAGAACCCTCT GCTGGCAGACGCAGCTGCTCTCGGTAAATGCTACAGGGTGATGGATTTGATCTGTGAGAAATGCATGAAA CAGAGAGACATGAATGAAGTGTTGGCTATGAAAATGCACTACATCAGCTGCATCTTTCAGAAATGCATTA CCTTCCTCAAAGAAGGAGAGAATAAGCTGGACACCCTGATCAGAAGCTTGTTGAAAGGCCGAGCGTCTGA TGGCTTCCCGGTGTATCAGGAAAAGATCATCAGAGAAAGCATCCGGAAGTTTCCATACTGTGAAGCCACA CTCCTACAGCAGCTGGTGCGAAGCATCGCTCCAGTTGAAATTGTAAGGCTCCTCTTTTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204185 protein sequence
Red=Cloning site Green=Tags(s) MGHIKKGELTQEEKELLEVIGKGTVQEAGRLLSSKNVHVNCLDENGMTPLMHAAYKGKLEMCKLLLRHGA DASCHQHEHGYTALMFAALSGNKDITWVMLEAGAETDVVNSVGRTAAQMAAFVGQHDCVAIINNFFPRER LDYYTKPQGLDKEPKLPPKLAGPLHKIITTTNLHPVKIVMLVSENPLLADAAALGKCYRVMDLICEKCMK QRDMNEVLAMKMHYISCIFQKCITFLKEGENKLDTLIRSLLKGRASDGFPVYQEKIIRESIRKFPYCEAT LLQQLVRSIAPVEIVRLLFC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC012275 |
| ORF Size | 900 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC012275, AAH12275 |
| RefSeq Size | 1525 bp |
| RefSeq ORF | 902 bp |
| Locus ID | 217473 |
| MW | 33.6 kDa |
| Gene Summary | May be involved in the trafficking of signaling proteins to the cilia.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Ankmy2 Prevents Smoothened-Independent Hyperactivation of the Hedgehog Pathway via Cilia-Regulated Adenylyl Cyclase Signaling
,Somatilaka, BN;Hwang, SH;Palicharla, VR;White, KA;Badgandi, H;Shelton, JM;Mukhopadhyay, S;,
Dev. Cell
,PubMed ID 32702291
[ANKMY2]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG204185 | Ankmy2 (tGFP-tagged) - Mouse ankyrin repeat and MYND domain containing 2 (cDNA clone MGC:18754 IMAGE:4010781) |
CNY 2,850.00 |
|
| MR204185L3 | Lenti ORF clone of Ankmy2 (Myc-DDK-tagged) - Mouse ankyrin repeat and MYND domain containing 2 (cDNA clone MGC:18754 IMAGE:4010781) |
CNY 4,750.00 |
|
| MR204185L4 | Lenti ORF clone of Ankmy2 (mGFP-tagged) - Mouse ankyrin repeat and MYND domain containing 2 (cDNA clone MGC:18754 IMAGE:4010781) |
CNY 4,750.00 |
