Crx (NM_007770) Mouse Tagged ORF Clone

CAT#: MR204176

  • TrueORF®

Crx (Myc-DDK-tagged) - Mouse cone-rod homeobox containing gene (Crx), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_007770" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,400.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


CRX Antibody - middle region
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms Crx1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR204176 representing NM_007770
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGCATATATGAACCCGGGGCCTCACTATTCAGTCAATGCCTTGGCTCTGAGTGGCCCCAATGTGG
ACCTGATGCACCAGGCTGTCCCATACTCAAGTGCCCCTAGGAAGCAGCGGCGGGAGCGGACCACATTCAC
CAGGAGCCAGCTGGAGGAGCTGGAGGCCCTGTTTGCCAAGACCCAGTACCCGGATGTGTATGCACGTGAG
GAGGTTGCTCTTAAGATCAATCTGCCTGAGTCCAGGGTCCAGGTCTGGTTCAAGAATCGTAGGGCGAAAT
GCAGACAGCAGCGACAGCAGCAGAAACAGCAACAGCAGCCCCCGGGGGCACAGACCAAGGCTCGTCCTGC
GAAGAGGAAGGCAGGGACATCCCCGAGACCCTCTACAGATGTTTGTACAGATCCTTTGGGCATCTCAGAT
TCTTACAGCCCATCTCTGCCTGGCCCCTCAGGTTCACCTACCACAGCAGTGGCCACCGTGTCCATTTGGA
GTCCAGCCTCAGAGGCTCCTTTGCCTGAGGCCCAGAGAGCCGGCCTAGTGGCTTCTGGGCCCTCTCTCAC
CTCAGCCCCTTATGCCATGACCTATGCCCCGGCTTCTGCTTTCTGTTCTTCCCCTTCAGCTTATGCATCT
CCAAGTTCCTATTTCAGTGGGTTGGATCCCTACCTTTCGCCCATGGTGCCCCAACTTGGGGGTCCGGCTC
TCAGCCCCCTCTCAGGCCCCTCTGTGGGGCCATCCCTGGCCCAGTCCCCCACCTCCTTGTCAGGCCAGAG
CTATAGCACCTACAGTCCTGTGGACAGCCTGGAATTCAAGGACCCCACAGGCACCTGGAAATTTACCTAC
AATCCCATGGACCCTCTGGACTACAAAGATCAGAGTGCCTGGAAGTTTCAGATCTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR204176 representing NM_007770
Red=Cloning site Green=Tags(s)

MMAYMNPGPHYSVNALALSGPNVDLMHQAVPYSSAPRKQRRERTTFTRSQLEELEALFAKTQYPDVYARE
EVALKINLPESRVQVWFKNRRAKCRQQRQQQKQQQQPPGAQTKARPAKRKAGTSPRPSTDVCTDPLGISD
SYSPSLPGPSGSPTTAVATVSIWSPASEAPLPEAQRAGLVASGPSLTSAPYAMTYAPASAFCSSPSAYAS
PSSYFSGLDPYLSPMVPQLGGPALSPLSGPSVGPSLAQSPTSLSGQSYSTYSPVDSLEFKDPTGTWKFTY
NPMDPLDYKDQSAWKFQIL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_007770
ORF Size 897 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_007770.4
RefSeq Size 2848 bp
RefSeq ORF 900 bp
Locus ID 12951
UniProt ID O54751
MW 32.8 kDa
Gene Summary Transcription factor that binds and transactivates the sequence 5'-TAATC[CA]-3' which is found upstream of several photoreceptor-specific genes, including the opsin genes. Acts synergistically with other transcription factors, such as NRL, RORB and RAX, to regulate photoreceptor cell-specific gene transcription. Essential for the maintenance of mammalian photoreceptors.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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