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Otud6b (NM_152812) Mouse Tagged ORF Clone
CAT＃: MR204060
- TrueORF®
Otud6b (Myc-DDK-tagged) - Mouse OTU domain containing 6B (Otud6b)
ORF Plasmid: tGFP
"NM_152812" in other vectors (4)
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CNY 2,400.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2600013N14Rik; AU015433 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR204060 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGGTCGTGGCTGAAGAGCTCGATGATGAGGAGCAGCTGGTGAGAAGGCATCGGAAGGAGAAGA AGGAGCTGCAAGCCAAAATTCAGGGAATGAAGAACGCTGTTCCCAAAAACGACAAAAAGAGGAGGAAGCA GCTCACGGAAGATGTTGCTAAATTGGAAAGAGAAATGGAGCAAAAACACAGGGAAGAACTGGAGCAATTG AAGCAATTGACTTTCAAGGACAGTAAGATAGATTCTGTTGCTGTTAACATTTCAAACTTGGTACTTGAGA ATCAACCACCTCGGATTTCAAAAGCACAAAAGAGACGGGAAAAGAAGGCTGCATTGGAAAAGGAGCGGGA AGAAAGGATAGCAGAGGCTGAAATTGAGAACTTATCTGGAGCCAGACATCTTGAGAGTGAAAAACTTGCT CAAATATTGGCAGCCAGAGAATTGGAAATTAAACAGATTCCATCTGATGGTCACTGTATGTATGGAGCAC TTGAGGATCAGCTGAGAGAACAAGACTGTGCGCTGACTGTGGCTTCCCTGAGGAGGCAGACTGCTGAGTA CATGCAAACCCATTCAGACGACTTCCTGCCGTTTCTAACAAACCCCAGCACAGGAGATATGTACACGCCA GAAGAGTTTGGGAAGTACTGTGACGATATTGTGAACACAGCGGCATGGGGCGGTCAGCTCGAGCTAAGAG CTTTGTCTCACATCTTACAAACACCAATCGAGATACTACAGGCAGATGCTCCTCCTATTATAGTCGGTGA AGAGTATCCGAGGAATCCATTAGTACTAGTATACATGAGGCACGCGTATGGCTTAGGAGAACATTATAAT TCTGTTACACGGTTGGTGAACTCAGCTACTGAAAATTGCAGC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR204060 protein sequence
Red=Cloning site Green=Tags(s) MEEVVAEELDDEEQLVRRHRKEKKELQAKIQGMKNAVPKNDKKRRKQLTEDVAKLEREMEQKHREELEQL KQLTFKDSKIDSVAVNISNLVLENQPPRISKAQKRREKKAALEKEREERIAEAEIENLSGARHLESEKLA QILAARELEIKQIPSDGHCMYGALEDQLREQDCALTVASLRRQTAEYMQTHSDDFLPFLTNPSTGDMYTP EEFGKYCDDIVNTAAWGGQLELRALSHILQTPIEILQADAPPIIVGEEYPRNPLVLVYMRHAYGLGEHYN SVTRLVNSATENCS SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_152812 |
| ORF Size | 885 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_152812.4 |
| RefSeq Size | 3311 bp |
| RefSeq ORF | 978 bp |
| Locus ID | 72201 |
| UniProt ID | Q8K2H2 |
| MW | 33.8 kDa |
| Gene Summary | Deubiquitinating enzyme that may play a role in the ubiquitin-dependent regulation of protein synthesis, downstream of mTORC1 (By similarity). May associate with the protein synthesis initiation complex and modify its ubiquitination to repress translation (By similarity). May also repress DNA synthesis and modify different cellular targets thereby regulating cell growth and proliferation (By similarity). May also play a role in proteasome assembly and function (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC207720 | Otud6b (untagged) - Mouse OTU domain containing 6B (Otud6b), (10ug) |
CNY 3,990.00 |
|
| MG204060 | Otud6b (tGFP-tagged) - Mouse OTU domain containing 6B (Otud6b) |
CNY 2,850.00 |
|
| MR204060L3 | Lenti ORF clone of Otud6b (Myc-DDK-tagged) - Mouse OTU domain containing 6B (Otud6b) |
CNY 4,750.00 |
|
| MR204060L4 | Lenti ORF clone of Otud6b (mGFP-tagged) - Mouse OTU domain containing 6B (Otud6b) |
CNY 4,750.00 |
