United States
Germany
Japan
United Kingdom
China
Igfbp3 (NM_008343) Mouse Tagged ORF Clone
CAT＃: MR204018
- TrueORF®
Igfbp3 (Myc-DDK-tagged) - Mouse insulin-like growth factor binding protein 3 (Igfbp3)
"NM_008343" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 3,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI649005; IGFBP-3; IGgfbp3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR204018 representing NM_008343
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATCCCGCGCGCCCCGCGCTCTGGGCGGCTGCGCTCACTGCCCTCACTCTGCTCCGCGG[AT]CCGC CGGTGGCGCGAGCTGGCGCGGGCGCGGTGGGCGCGGGCCCCGTGGTGCGCTGCGAACCGTGCGACGCGCG TGCGCTGTCCCAGTGCGCGCCTCCGCCCACTGCGCCCGCGTGCACGGAGCTGGTGCGAGAGCCCGGCTGC GGCTGCTGCCTGACTTGCGCGCTGCGCGAAGGCGACGCGTGCGGCGTCTACACGGAGCGCTGTGGCACCG GCCTCCGCTGCCAGCCGCGGCCCGCGGAGCAGTACCCGCTGAGGGCGCTGCTGAATGGCCGCGGGTTCTG CGCCAACGCCAGCGCTGCAGGCAGCCTAAGCACCTACCTCCCCTCCCAACCTGCTCCAGGAAACATCAGT GAGTCCGAGGAGGAGCACAATGCTGGGAGTGTGGAAAGCCAGGTTGTCCCCAGCACACACCGAGTGACCG ATTCCAAGTTCCATCCACTCCATGCCAAGATGGATGTCATCAAAAAAGGCCACGCCAGGGACAGCCAGCG CTACAAAGTTGACTATGAGTCTCAAAGCACAGACACCCAGAACTTCTCCTCCGAGTCTAAGCGGGAGACA GAATACGGTCCCTGCCGCAGAGAAATGGAGGACACACTGAATCATCTGAAGTTCCTCAATGTGCTGAGTC CCAGAGGCGTCCACATCCCAAACTGTGACAAGAAGGGGTTCTATAAGAAGAAGCAGTGCCGCCCTTCCAA AGGCAGAAAGCGGGGCTTCTGCTGGTGTGTGGACAAGTATGGGCAGCCCTTGCCAGGCTACGACACCAAG GGGAAAGACGACGTACATTGCCTCAGCGTGCAGAGCCAG ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA >MR204018 representing NM_008343
Red=Cloning site Green=Tags(s) MHPARPALWAAALTALTLLRXXPPVARAGAGAVGAGPVVRCEPCDARALSQCAPPPTAPACTELVREPGC GCCLTCALREGDACGVYTERCGTGLRCQPRPAEQYPLRALLNGRGFCANASAAGSLSTYLPSQPAPGNIS ESEEEHNAGSVESQVVPSTHRVTDSKFHPLHAKMDVIKKGHARDSQRYKVDYESQSTDTQNFSSESKRET EYGPCRREMEDTLNHLKFLNVLSPRGVHIPNCDKKGFYKKKQCRPSKGRKRGFCWCVDKYGQPLPGYDTK GKDDVHCLSVQSQ TRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-NotI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008343 |
| ORF Size | 879 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008343.2, NP_032369.2 |
| RefSeq Size | 2456 bp |
| RefSeq ORF | 879 bp |
| Locus ID | 16009 |
| UniProt ID | P47878 |
| MW | 32.1 kDa |
| Gene Summary | IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203300 | Igfbp3 (untagged) - Mouse insulin-like growth factor binding protein 3 (Igfbp3), (10ug) |
CNY 2,400.00 |
|
| MR204018L3 | Lenti ORF clone of Igfbp3 (Myc-DDK-tagged) - Mouse insulin-like growth factor binding protein 3 (Igfbp3) |
CNY 4,750.00 |
|
| MR204018L4 | Lenti ORF clone of Igfbp3 (mGFP-tagged) - Mouse insulin-like growth factor binding protein 3 (Igfbp3) |
CNY 4,750.00 |
