Lime1 (NM_023684) Mouse Tagged ORF Clone
CAT#: MR203578
- TrueORF®
Lime1 (Myc-DDK-tagged) - Mouse Lck interacting transmembrane adaptor 1 (Lime1)
ORF Plasmid: tGFP
"NM_023684" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2810038K19Rik; LIME |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR203578 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCCACCAGTGCCTTCAGCCCCACTAGCTCTCTGGGTCCTGGGCTGCTTCTCCCTGCTCCTTTGGC TATGGGCACTGTGCACAGCCTGCCACAGGAAGAGAGCTCAGAGGCAGCAAACTGGGCTGCAGGACAGTTT GGTACCAGTGGAAATGCCACTGCTGAGACAGACCCACCTCTGCTCCCTCAGCAAGTCTGACACCAGACTG CATGAGCTGCACCGAGGCCCACGCTCCAGCATAGCCCCACGGCCTGCTAGCATGGATCTTCTGCATCCAC GCTGGCTAGAGATGTCCAGGGGCAGCACCAGGTCTCAGGTACCCAACTCTGCCTTCCCACCACGGCAGCT GCCCAGGGCCCCTCCTGCTGCTCCTGCAACTGCACCTTCTACTAGCTCTGAGGCCACTTATTCCAATGTG GGGCTGGCTGCAATTCCCAGGGCCAGCCTGGCTGCTAGCCCTGTGGTGTGGGCTGGAACACAGCTGACCA TTAGTTGTGCCAGGCTTGGTCCTGGGGCCGAATATGCCTGCATCCAGAAGCACAAAGGGACAGAGCAAGG CTGCCAAGAATTGCAGCAGAAGGCTAAGGTGATCCCAGCTACTCAGATGGACGTCCTATACTCTAGGGTT TGCAAGCCTAAAAGAAGAGACCCAAGACCCGTCACAGACCAGCTGAACCTGCAGGATGGGAGAACAAGTC TGCCGCTTGGGAGTGATGTGGAGTACGAGGCCATCAATCTCAGGGGCCAGGATATGAAGCAAGGGCCCTT AGAAAATGTGTATGAAAGCATCAAGGAGATGGGCCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203578 protein sequence
Red=Cloning site Green=Tags(s) MRPPVPSAPLALWVLGCFSLLLWLWALCTACHRKRAQRQQTGLQDSLVPVEMPLLRQTHLCSLSKSDTRL HELHRGPRSSIAPRPASMDLLHPRWLEMSRGSTRSQVPNSAFPPRQLPRAPPAAPATAPSTSSEATYSNV GLAAIPRASLAASPVVWAGTQLTISCARLGPGAEYACIQKHKGTEQGCQELQQKAKVIPATQMDVLYSRV CKPKRRDPRPVTDQLNLQDGRTSLPLGSDVEYEAINLRGQDMKQGPLENVYESIKEMGL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_023684 |
ORF Size | 810 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_023684.2, NP_076173.1 |
RefSeq Size | 1987 bp |
RefSeq ORF | 810 bp |
Locus ID | 72699 |
UniProt ID | Q9EQR5 |
MW | 29.6 kDa |
Gene Summary | Involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and TCR (T-cell antigen receptor)-mediated T-cell signaling in T-cells. In absence of TCR signaling, may be involved in CD4-mediated inhibition of T-cell activation. Couples activation of these receptors and their associated kinases with distal intracellular events such as calcium mobilization or MAPK activation through the recruitment of PLCG2, GRB2, GRAP2, and other signaling molecules.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC204311 | Lime1 (untagged) - Mouse Lck interacting transmembrane adaptor 1 (Lime1), (10ug) |
CNY 2,400.00 |
|
MG203578 | Lime1 (tGFP-tagged) - Mouse Lck interacting transmembrane adaptor 1 (Lime1) |
CNY 2,850.00 |
|
MR203578L3 | Lenti ORF clone of Lime1 (Myc-DDK-tagged) - Mouse Lck interacting transmembrane adaptor 1 (Lime1) |
CNY 4,750.00 |
|
MR203578L4 | Lenti ORF clone of Lime1 (mGFP-tagged) - Mouse Lck interacting transmembrane adaptor 1 (Lime1) |
CNY 4,750.00 |