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Med8 (NM_020000) Mouse Tagged ORF Clone
CAT＃: MR203549
- TrueORF®
Med8 (Myc-DDK-tagged) - Mouse mediator of RNA polymerase II transcription, subunit 8 homolog (yeast) (Med8), transcript variant 1
"NM_020000" in other vectors (3)
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CNY 2,400.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2210021A15Rik; AB041805; ARC32 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR203549 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAGGGAGGAGAAGCAGCTTGAGGCATCATTAGATGCCCTGCTGAATCAAGTGGCCGATCTGAAGA ATTCACTGGGGAGTTTCATTTACAAGTTGGAAAACGAGTATGACCGGCTGACCTGGCCCTCTGTCCTGGA CAGCTTTGCATTGCTTTCTGGACAGTTGAACACTCTGAACAAGGTCTTAAAGCATGAGAAGACGCCACTG TTCCGAAACCAGGTCATCATCCCTCTGGTGCTGTCCCCAGACCGAGACGAAGACCTCATGCGTCAAACTG AAGGACGAGTGCCTGTTTTCAGCCATGAGGTCGTCCCTGACCACTTGAGAACCAAGCCTGACCCTGAAGT TGAAGAGCAGGAGAAGCAACTGACAACAGATGCTGCCAGAATTGGTGCTGATGCAGCTCAGAAGCAGATC CAGAGCTTGAATAAAATGTGCTCAAACCTTTTAGAGAAAATCAGCAAAGAAGAACGAGAATCAGAGAGCG GAGGTCTCCGGCCAAACAAGCAGACTTTCAACCCTGGAGACACCAATGCCTTAGTAGCAGCCGTTGCCTT TGGGAAGGGGCTATCTAATTGGCGACCTTCAGGTAGCAGTGGTCCTGGGCAACCCGGTCAGCCAGGAGCT GGTACAATCCTTGCAGGGGCCTCAGGATTACCGCAGGTTCAGATGCCAGGTGCTCCAAACCAGCAGCAGC CAATGCTCAGTGGAGTCCAGATGGCTCAGGCAGGTCAACCAGGGAAAATGCCAAGTGGAATAAAAACCAA CATCAAGTCAGCTTCAATGCATCCGTACCAGCGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203549 protein sequence
Red=Cloning site Green=Tags(s) MQREEKQLEASLDALLNQVADLKNSLGSFIYKLENEYDRLTWPSVLDSFALLSGQLNTLNKVLKHEKTPL FRNQVIIPLVLSPDRDEDLMRQTEGRVPVFSHEVVPDHLRTKPDPEVEEQEKQLTTDAARIGADAAQKQI QSLNKMCSNLLEKISKEERESESGGLRPNKQTFNPGDTNALVAAVAFGKGLSNWRPSGSSGPGQPGQPGA GTILAGASGLPQVQMPGAPNQQQPMLSGVQMAQAGQPGKMPSGIKTNIKSASMHPYQR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020000 |
| ORF Size | 807 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020000.2, NM_020000.3, NP_064384.2 |
| RefSeq Size | 1887 bp |
| RefSeq ORF | 807 bp |
| Locus ID | 80509 |
| UniProt ID | Q9D7W5 |
| MW | 29.2 kDa |
| Gene Summary | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203111 | Med8 (untagged) - Mouse mediator of RNA polymerase II transcription, subunit 8 homolog (yeast) (Med8), transcript variant 1, (10ug) |
CNY 2,400.00 |
|
| MR203549L3 | Lenti ORF clone of Med8 (Myc-DDK-tagged) - Mouse mediator of RNA polymerase II transcription, subunit 8 homolog (yeast) (Med8), transcript variant 1 |
CNY 4,750.00 |
|
| MR203549L4 | Lenti ORF clone of Med8 (mGFP-tagged) - Mouse mediator of RNA polymerase II transcription, subunit 8 homolog (yeast) (Med8), transcript variant 1 |
CNY 4,750.00 |
