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Klk1b8 (NM_008457) Mouse Tagged ORF Clone
CAT＃: MR203410
- TrueORF®
Klk1b8 (Myc-DDK-tagged) - Mouse kallikrein 1-related peptidase b8 (Klk1b8)
ORF Plasmid: tGFP
"NM_008457" in other vectors (4)
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CNY 2,400.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | kallikrein; Klk; Klk8; mGK-8; Nrpn; TADG1; TADG14 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR203410 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGTTCCTGATCCTGTTCCTAGCCCTGTCCCTAGGAGGGATTGATGCTGCACCTCCTCTCCAGTCTC GGGTGGTTGGAGGATTTAACTGTGAGAAGAATTCCCAACCCTGGCAGGTGGCTGTGTATGACAACAAGGA ACACATTTGTGGGGGAGTCCTGTTGGAACGCAACTGGGTTCCCACAGCTGCCCACTGCTATGTCGACCAG TATGAGGTTTGGCTGGGCAAAAACAAGTTATTCCAAGAGGAACCCTCTGCTCAACACCGATTGGTCAGCA AAAGCTTCCCTCACCCTGGCTTCAACATGAGCCTCCTGACACTCAAAGAGATACCTCCTGGGGCTGACTT CAGCAATGACCTGATGCTGCTCCGCCTCAGCAAGCCTGCTGACATCACAGATGCTGTGAAGCCCATCACC CTGCCCACGAAGGAGTCAAAACTGGGGAGCACATGCCTAGCCTCAGGCTGGGGCAGCATTACACCCACGA AATGGCAAAAGCCAGATGATCTCCAGTGTGTGTTCCTCAAGCTCCTGCCTATTAAGAACTGTATAGAAAA CCACAATGTGAAGGTGACAGATGTCATGCTGTGTGCAGGAGAGATGAGTGGAGGCAAAAACATTTGCAAG GGTGACTCCGGAGGCCCATTGATCTGTGATAGTGTTCTCCAAGGTATCACATCAACAGGCCCTATACCAT GCGGTAAACCTGGTGTACCAGCCATGTACACCAACCTTATTAAGTTCAACTCCTGGATAAAAGATACTAT GACGAAAAATTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203410 protein sequence
Red=Cloning site Green=Tags(s) MRFLILFLALSLGGIDAAPPLQSRVVGGFNCEKNSQPWQVAVYDNKEHICGGVLLERNWVPTAAHCYVDQ YEVWLGKNKLFQEEPSAQHRLVSKSFPHPGFNMSLLTLKEIPPGADFSNDLMLLRLSKPADITDAVKPIT LPTKESKLGSTCLASGWGSITPTKWQKPDDLQCVFLKLLPIKNCIENHNVKVTDVMLCAGEMSGGKNICK GDSGGPLICDSVLQGITSTGPIPCGKPGVPAMYTNLIKFNSWIKDTMTKNS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008457 |
| ORF Size | 786 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008457.1, NM_008457.2, NM_008457.3, NP_032483.1 |
| RefSeq Size | 848 bp |
| RefSeq ORF | 786 bp |
| Locus ID | 16624 |
| UniProt ID | P07628 |
| MW | 28.5 kDa |
| Gene Summary | This gene encodes a member of the kallikrein subfamily of serine proteases that are involved in diverse physiological functions such as skin desquamation, tooth enamel formation, seminal liquefaction, synaptic neural plasticity and brain function. The encoded preproprotein undergoes proteolytic cleavage of the activation peptide to generate the functional enzyme. This gene is located in a cluster of several related kallikrein genes on chromosome 7. [provided by RefSeq, Feb 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201430 | Klk1b8 (untagged) - Mouse kallikrein 1-related peptidase b8 (Klk1b8), (10ug) |
CNY 2,400.00 |
|
| MG203410 | Klk1b8 (tGFP-tagged) - Mouse kallikrein 1-related peptidase b8 (Klk1b8) |
CNY 2,850.00 |
|
| MR203410L3 | Lenti ORF clone of Klk1b8 (Myc-DDK-tagged) - Mouse kallikrein 1-related peptidase b8 (Klk1b8) |
CNY 4,750.00 |
|
| MR203410L4 | Lenti ORF clone of Klk1b8 (mGFP-tagged) - Mouse kallikrein 1-related peptidase b8 (Klk1b8) |
CNY 4,750.00 |
