Dpm1 (NM_010072) Mouse Tagged ORF Clone
CAT#: MR203382
- TrueORF®
Dpm1 (Myc-DDK-tagged) - Mouse dolichol-phosphate (beta-D) mannosyltransferase 1 (Dpm1)
ORF Plasmid: tGFP
"NM_010072" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AI118379; AI194292 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR203382 representing NM_010072
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCCACGGGGGCGAGTCGCAGTCTTGCTGCGTCTCCGCGGCCGCCGCAGGGCCGCTCCTCGCGAC AGGACAAGTACTCGGTGCTGTTGCCCACCTACAACGAACGGGAGAACCTGCCGCTCATCGTGTGGCTGCT GGTGAAGAGCTTCTCCGAGAGTGCAATCAACTATGAAATTATAATCATAGATGATGGAAGCCCAGACGGG ACAAGAGAAGTTGCTGAACAACTGGCAGAGATCTATGGGCCCGACAGAATTCTCCTAAGACCACGAGAGA AAAAGCTGGGTCTCGGAACTGCCTACATTCATGGAATCAAACACGCCACGGGGAACTATGTGATCATCAT GGATGCTGACCTCTCCCACCATCCCAAATTTATTCCTGAATTTATCAGGAAACAAAAGGAGGGTAATTTT GATATTGTCTCTGGAACTCGATACAAGGGGAATGGGGGTGTTTATGGATGGGATTTGAAAAGAAAGATAA TCAGCCGTGGGGCCAATTTTATAACTCAAATTCTGCTGAGACCTGGAGCGTCAGACTTAACAGGAAGCTT CAGATTATACCGAAAAGAAGTTCTACAGAAATTAATAGAAAAATGTGTCTCCAAAGGCTATGTCTTTCAG ATGGAAATGATTGTTCGAGCAAGACAGATGAATTACACTATTGGTGAGGTTCCAATTTCATTTGTGGATC GAGTTTATGGTGAATCCAAGTTGGGAGGAAATGAAATAGTCTCGTTCCTGAAAGGGTTATTGACTCTCTT TGCTACCACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203382 representing NM_010072
Red=Cloning site Green=Tags(s) MASTGASRSLAASPRPPQGRSSRQDKYSVLLPTYNERENLPLIVWLLVKSFSESAINYEIIIIDDGSPDG TREVAEQLAEIYGPDRILLRPREKKLGLGTAYIHGIKHATGNYVIIMDADLSHHPKFIPEFIRKQKEGNF DIVSGTRYKGNGGVYGWDLKRKIISRGANFITQILLRPGASDLTGSFRLYRKEVLQKLIEKCVSKGYVFQ MEMIVRARQMNYTIGEVPISFVDRVYGESKLGGNEIVSFLKGLLTLFATT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010072 |
ORF Size | 780 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010072.4, NP_034202.1 |
RefSeq Size | 2293 bp |
RefSeq ORF | 783 bp |
Locus ID | 13480 |
UniProt ID | O70152 |
MW | 29.6 kDa |
Gene Summary | Transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O-mannosylation of proteins; catalytic subunit of the dolichol-phosphate mannose (DPM) synthase complex.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208406 | Dpm1 (untagged) - Mouse dolichol-phosphate (beta-D) mannosyltransferase 1 (Dpm1), (10ug) |
CNY 3,990.00 |
|
MG203382 | Dpm1 (tGFP-tagged) - Mouse dolichol-phosphate (beta-D) mannosyltransferase 1 (Dpm1) |
CNY 2,850.00 |
|
MR203382L3 | Lenti ORF clone of Dpm1 (Myc-DDK-tagged) - Mouse dolichol-phosphate (beta-D) mannosyltransferase 1 (Dpm1) |
CNY 4,750.00 |
|
MR203382L4 | Lenti ORF clone of Dpm1 (mGFP-tagged) - Mouse dolichol-phosphate (beta-D) mannosyltransferase 1 (Dpm1) |
CNY 4,750.00 |