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Trh (NM_009426) Mouse Tagged ORF Clone
CAT＃: MR203300
- TrueORF®
Trh (Myc-DDK-tagged) - Mouse thyrotropin releasing hormone (Trh)
ORF Plasmid: tGFP
"NM_009426" in other vectors (4)
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CNY 1,416.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Pro-TRH; Trf |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR203300 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGGACCTTGGCTGATGATGGCTCTGGCTTTGATCTTCGTGCTAACTGGTATCCCCAAATCCTGCG CCTTGCTGGAAGCAGCCCAGGAGGAAGGTGCTGTGACTCCTGACCTTCCAGGCCTGGAGAAAGTCCAGGT CCGGCCAGAACGTCGATTCTTGAGGAAAGACCTCCAGCGTGTGCGAGGGGACCTTGGTGCTGCCTTAGAT TCCTGGATCACAAAACGCCAGCATCCGGGCAAAAGGGAGGAGAAGTTGGAAGACGAAGCCGAAGAGAGGG GAGATTTGGGAGAAGTGGGAGCCTGGAGACCCCACAAACGACAGCACCCTGGCCGACGTGCCAACCAAGA CAAGGATTCATGGTCAGATGAGGGGGACAGTGACTGGCTGCCCCCGTCCTGGCTACCAGATTTCTTTCTG GATTCCTGGTTCTCAGATGCCCCCCAAGTCAAGCGGCAGCACCCTGGCAGGCGATCCTTCCCCTGGATGG AGTCTGATGTCACCAAGAGGCAGCATCCAGGCCGGAGGTTCATAGATCCTGAGCTCCAAAGAAGCTGGGA AGAAACAGAGGGAGAGGAGGGTGGCTTAATGCCTGAGAAACGTCAGCATCCTGGCAAAAGGGCAGTGGGT CACCCTTGTGGGCCCCAGGGGATTTGTGGCCAAACAGGCCTGCTCCAGCTTCTAGGTGACCTGAGTAGGG GCCAGGAGACCCTGGCAAAGCAAACCCCACAACTGGAAGCCTGGGTCAGGGAACCTCTGGAGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203300 protein sequence
Red=Cloning site Green=Tags(s) MQGPWLMMALALIFVLTGIPKSCALLEAAQEEGAVTPDLPGLEKVQVRPERRFLRKDLQRVRGDLGAALD SWITKRQHPGKREEKLEDEAEERGDLGEVGAWRPHKRQHPGRRANQDKDSWSDEGDSDWLPPSWLPDFFL DSWFSDAPQVKRQHPGRRSFPWMESDVTKRQHPGRRFIDPELQRSWEETEGEEGGLMPEKRQHPGKRAVG HPCGPQGICGQTGLLQLLGDLSRGQETLAKQTPQLEAWVREPLEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009426 |
| ORF Size | 768 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009426.1, NM_009426.2, NM_009426.3, NP_033452.2 |
| RefSeq Size | 1400 bp |
| RefSeq ORF | 771 bp |
| Locus ID | 22044 |
| UniProt ID | Q62361 |
| MW | 29.1 kDa |
| Gene Summary | This gene encodes a member of the thyrotropin-releasing hormone family. Cleavage of the encoded proprotein releases mature thyrotropin-releasing hormone, which is a tripeptide hypothalamic regulatory hormone. The mouse proprotein contains five thyrotropin-releasing hormone tripeptides. Thyrotropin-releasing hormone is involved in the regulation and release of thyroid-stimulating hormone, as well as prolactin. Disruption of this gene results in hypothyroidism, elevated thyroid-stimulating hormone levels, and hyperglycemia. [provided by RefSeq, Apr 2013] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Differential regulation of thyrotropin-releasing hormone mRNA expression in the paraventricular nucleus and dorsomedial hypothalamus in OLETF rats
,Zhang, N;Zhang, HY;Bi, SA;Moran, TH;Bi, S;,
Neurosci. Lett.
,PubMed ID 30902570
[TRH]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202032 | Trh (untagged) - Mouse thyrotropin releasing hormone (Trh), (10ug) |
CNY 2,000.00 |
|
| MG203300 | Trh (tGFP-tagged) - Mouse thyrotropin releasing hormone (Trh) |
CNY 2,850.00 |
|
| MR203300L3 | Lenti ORF clone of Trh (Myc-DDK-tagged) - Mouse thyrotropin releasing hormone (Trh) |
CNY 4,750.00 |
|
| MR203300L4 | Lenti ORF clone of Trh (mGFP-tagged) - Mouse thyrotropin releasing hormone (Trh) |
CNY 4,750.00 |
