United States
Germany
Japan
United Kingdom
China
Hacd2 (NM_023587) Mouse Tagged ORF Clone
CAT＃: MR203263
- TrueORF®
Ptplb (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase-like (proline instead of catalytic arginine), member b (Ptplb)
ORF Plasmid: tGFP
"NM_023587" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 2,081.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 6330408J20Rik; AI255777; AI481689; Hcad2; Ptplb |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR203263 representing NM_023587
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCAGCGGCGGCGACTGCAGCGACCAAGGGGAATGGGGGCGGCAGTGGCCGAGTCGGGGCTGGCG ACAGCAGCGGCGCGCGGAAGAAGAAGGGCCCCGGGCCTGTGGCCACGGCGTACCTGGTCATCTACAATGT GGTGATGACGGCGGGGTGGCTGGTGATAGCAGTTGGGCTGGTGAGAGCATACCTGGCTAAGGGGAGCTAC CATAGCCTTTATTATTCAATAGAAAGGCCTTTGAAGTTCTTCCAGACAGGAGCCTTACTGGAGATTTTAC ATTGTGCTATAGGGATTGTGCCATCTTCTGTTGTCCTGACTTCTTTCCAGGTGATGTCAAGAGTTTTCCT AATATGGGCAGTGACACATAGTGTCAAAGAGGTGCAGAGTGAAGACAGTGTACTTCTGTTTGTCATTGCC TGGACAATCACGGAAATTATCCGTTACTCCTTTTACACATTCAGTCTATTAAACCATCTGCCTTATATCA TCAAATGGGCCAGGTACACACTTTTCATCGTGCTGTACCCAATGGGAGTGACAGGAGAGCTCCTCACAAT ATACGCAGCTCTGCCCTTTGTCCGGCAGGCCGGCCTGTACTCCATCAGCTTACCTAACAAATACAACTTC TCCTTTGATTACCATGCATTCCTGATTCTGATCATGATCTCCTACATTCCACTGTTCCCCCAGTTGTACT TCCACATGATACACCAGAGAAGAAAGGTCCTTTCTCACACCGAAGAACATAAGAAGTTTGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203263 representing NM_023587
Red=Cloning site Green=Tags(s) MAAAAATAATKGNGGGSGRVGAGDSSGARKKKGPGPVATAYLVIYNVVMTAGWLVIAVGLVRAYLAKGSY HSLYYSIERPLKFFQTGALLEILHCAIGIVPSSVVLTSFQVMSRVFLIWAVTHSVKEVQSEDSVLLFVIA WTITEIIRYSFYTFSLLNHLPYIIKWARYTLFIVLYPMGVTGELLTIYAALPFVRQAGLYSISLPNKYNF SFDYHAFLILIMISYIPLFPQLYFHMIHQRRKVLSHTEEHKKFE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_023587 |
| ORF Size | 762 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_023587.2, NP_076076.2 |
| RefSeq Size | 3603 bp |
| RefSeq ORF | 765 bp |
| Locus ID | 70757 |
| UniProt ID | Q9D3B1 |
| MW | 28.9 kDa |
| Gene Summary | Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204564 | Ptplb (untagged) - Mouse protein tyrosine phosphatase-like (proline instead of catalytic arginine), member b (Ptplb), (10ug) |
CNY 2,000.00 |
|
| MG203263 | Ptplb (tGFP-tagged) - Mouse protein tyrosine phosphatase-like (proline instead of catalytic arginine), member b (Ptplb) |
CNY 2,850.00 |
|
| MR203263L3 | Lenti ORF clone of Ptplb (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase-like (proline instead of catalytic arginine), member b (Ptplb) |
CNY 4,750.00 |
|
| MR203263L4 | Lenti ORF clone of Ptplb (mGFP-tagged) - Mouse protein tyrosine phosphatase-like (proline instead of catalytic arginine), member b (Ptplb) |
CNY 4,750.00 |
