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Mpdu1 (BC048871) Mouse Tagged ORF Clone
CAT＃: MR203128
- TrueORF®
Mpdu1 (Myc-DDK-tagged) - Mouse mannose-P-dolichol utilization defect 1 (cDNA clone MGC:58915 IMAGE:5364408)
ORF Plasmid: tGFP
"BC048871" in other vectors (3)
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CNY 2,400.00
CNY 6,365.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SL15, LEC35 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR203128 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGGGAGGCCGACGGGCCGTTCAAAGGGCTGCTGGTGCCAATTCTTTTACCTGAGAAATGTTACG ACCAGCTCTTCGTGCAATGGGACTTGCTTCATGTTCCCTGCCTCAAGATTCTCCTCAGCAAAGGCCTCGG GCTGGGCATCGTGGCTGGGTCACTTCTCGTCAAGCTGCCCCAGGTATTTAAACTCTTGGGAGCCAAGAGT GCAGAAGGACTGAGTCTCCAGTCAGTAATGCTGGAGCTAGTGGCACTGACCGGAACCGTGGTCTACAGCA TCACCAACAACTTCCCCTTCAGCTCTTGGGGTGAAGCGCTGTTCCTGACACTCCAGACCGTTGCCATCTG CTTCCTGGTCATGCACTACAGAGGAGAGACCGTGAAAGGAGTCGCTTTCCTTGCCTGCTATGCCATGGTC CTGCTGGCGCTGCTCTCCCCGCTCACGCCTCTGGCTGTAGTCACTCTGCTCCAGGCCTCCAATGTACCTG CCGTGGTGGTGGGGAAGTTGCTTCAGGCAGCCACTAACTACCGCAACGGACACACAGGCCAGCTTTCAGC CATTACAGTGTTTATGCTGTTTGGGGGCTCCTTGGCCCGAATCTTCACTTCTGTTCAGGAAACTGGAGAC CCCCTCATGGCTGGAGTCTTTGTGGTCTCTTCTCTCTGCAATGGCCTCATTGCTGCCCAGGTCCTCTTCT ACTGGAACGCAAAGGCTCCCCACAAACAGAAAAAGGAGCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203128 protein sequence
Red=Cloning site Green=Tags(s) MAGEADGPFKGLLVPILLPEKCYDQLFVQWDLLHVPCLKILLSKGLGLGIVAGSLLVKLPQVFKLLGAKS AEGLSLQSVMLELVALTGTVVYSITNNFPFSSWGEALFLTLQTVAICFLVMHYRGETVKGVAFLACYAMV LLALLSPLTPLAVVTLLQASNVPAVVVGKLLQAATNYRNGHTGQLSAITVFMLFGGSLARIFTSVQETGD PLMAGVFVVSSLCNGLIAAQVLFYWNAKAPHKQKKEQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC048871 |
| ORF Size | 741 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC048871, AAH48871 |
| RefSeq Size | 1255 bp |
| RefSeq ORF | 743 bp |
| Locus ID | 24070 |
| MW | 26.4 kDa |
| Gene Summary | This gene encodes a member of the PQ-loop superfamily. A similar gene in human encodes a protein that is required for monosaccharide-P-dolichol-dependent glycosyltransferase reactions, and disruption of this gene is the cause of congenital disorder of glycosylation (CDG) type 1F, a disease linked to defects in protein N-glycosylation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG203128 | Mpdu1 (tGFP-tagged) - Mouse mannose-P-dolichol utilization defect 1 (cDNA clone MGC:58915 IMAGE:5364408) |
CNY 2,850.00 |
|
| MR203128L3 | Lenti ORF clone of Mpdu1 (Myc-DDK-tagged) - Mouse mannose-P-dolichol utilization defect 1 (cDNA clone MGC:58915 IMAGE:5364408) |
CNY 4,750.00 |
|
| MR203128L4 | Lenti ORF clone of Mpdu1 (mGFP-tagged) - Mouse mannose-P-dolichol utilization defect 1 (cDNA clone MGC:58915 IMAGE:5364408) |
CNY 4,750.00 |
