Rnf182 (NM_183204) Mouse Tagged ORF Clone
CAT#: MR203122
- TrueORF®
Rnf182 (Myc-DDK-tagged) - Mouse ring finger protein 182 (Rnf182)
ORF Plasmid: tGFP
"NM_183204" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | C630023L15Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR203122 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCCAGCCGCTGGAAGAGCCTGCGGAGTCTCAGGCCTCGGATGAGCTCGAGTGTAAGATCTGTT ACAATCGGTACAACCTGAAGCAGAGGAAGCCCAAGGTTCTGGAGTGTTGTCACAGGGTCTGCGCCAAATG CCTCTACAAGATCATAGACTTTGGGGACTCTCCCCAGGGGGTCATCGTCTGTCCCTTCTGCAGGTTTGAG ACCTGTCTGCCGGATGACGAAGTCAGCAGCCTGCCCGATGACAACAACATCCTTGTAAACTTGACCTGTG GGGGCAAAGGGAAGAAATGCCTGCCTGAGAACCCCACTGAGCTGTTGCTCACTCCCAAGAGGCTGGCTTC CCTTGTCAGCCCTTCCCACACGTCCTCTAATTGCTTGGTTATCACCATCATGGAGGTGCAGAGAGAGAGC TCTCCATCACTCAGCTCCACCCCCGTGGTAGAATTCTACAGGCCAGCCAGTTTCGACTCTGTCACCACCG TGTCCCATAACTGGACGGTGTGGAACTGCACCTCCTTGCTCTTTCAGACGTCCATCCGGGTGTTAGTGTG GCTGCTAGGTTTGCTGTACTTCAGCTCCTTGCCCTTAGGGATCTACTTACTGGTGTCTAAGAAAGTCACC CTGGGGGTGGTCTTCGTTAGCCTTGTCCCCTCTAGCCTTGTCATCCTGATGGTGTATGGTTTTTGCCAAT GCGTTTGTCATGAATTTCTAGACTGTATGGCACTTCCTTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203122 protein sequence
Red=Cloning site Green=Tags(s) MASQPLEEPAESQASDELECKICYNRYNLKQRKPKVLECCHRVCAKCLYKIIDFGDSPQGVIVCPFCRFE TCLPDDEVSSLPDDNNILVNLTCGGKGKKCLPENPTELLLTPKRLASLVSPSHTSSNCLVITIMEVQRES SPSLSSTPVVEFYRPASFDSVTTVSHNWTVWNCTSLLFQTSIRVLVWLLGLLYFSSLPLGIYLLVSKKVT LGVVFVSLVPSSLVILMVYGFCQCVCHEFLDCMALPS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_183204 |
ORF Size | 744 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_183204.4, NP_899027.1 |
RefSeq Size | 3456 bp |
RefSeq ORF | 744 bp |
Locus ID | 328234 |
UniProt ID | Q8C432 |
MW | 27.4 kDa |
Gene Summary | E3 ubiquitin-protein ligase that mediates the ubiquitination of ATP6V0C and targets it to degradation via the ubiquitin-proteasome pathway.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC214519 | Rnf182 (untagged) - Mouse ring finger protein 182 (Rnf182), (10ug) |
CNY 3,990.00 |
|
MG203122 | Rnf182 (tGFP-tagged) - Mouse ring finger protein 182 (Rnf182) |
CNY 2,850.00 |
|
MR203122L3 | Lenti ORF clone of Rnf182 (Myc-DDK-tagged) - Mouse ring finger protein 182 (Rnf182) |
CNY 4,750.00 |
|
MR203122L4 | Lenti ORF clone of Rnf182 (mGFP-tagged) - Mouse ring finger protein 182 (Rnf182) |
CNY 4,750.00 |