Kcnmb2 (NM_028231) Mouse Tagged ORF Clone

CAT#: MR202855

  • TrueORF®

Kcnmb2 (Myc-DDK-tagged) - Mouse potassium large conductance calcium-activated channel, subfamily M, beta member 2 (Kcnmb2)

ORF Plasmid: DDK tGFP



  "NM_028231" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 1,416.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 2700049B16Rik; 3110031N04Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR202855 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTATATGGACCAGTGGCCGGACCTCTTCATCTTACAGACAGGACGAGAAAAGAAATATCTACCAGA
AAATCAGGGACCATGACCTCCTGGACAAAAGGAAAACTGTGACAGCTCTGAAGGCTGGGGAGGACCGGGC
CATCCTGCTCGGCCTGGCCATGATGGTGTGCTCCATCATGATGTACTTCCTGCTGGGAATCACACTGCTG
CGCTCCTACATGCAGAGCGTGTGGACAGAAGAAGCCCAGTGTGCCCTGCTGAATGTGTCAATCACAGAAA
CGTTTAACTGTTCCTTCAGCTGTGGGCCCGACTGTTGGAAGCTCTCTCAGTACCCTTGCCTGCAGGTGTA
CGTGAACCTGACATCTTCCGGGGAGAGGCTCCTCCTCTACCACACGGAAGAGACCATGAAGATCAATCAA
AAGTGCTCCTATATTCCTAAGTGTGGAAACAACTTTGAGGAGTCCATGTCTCTCGTGAGTGTCGTCATGG
AAAACTTCAGGAGACACCAACACTTCCCCTGCTATTCTGACCCAGAAGGAAACCAGAAGAGTGTCATCCT
GACCAAACTCTACAGCTCCAATGTGCTGTTCCATTCTCTCTTCTGGCCAACTTGTATGATGGCTGGGGGT
GTGGCAATCGTTGCTATGGTGAAACTAACTCAGTACCTCTCCCTGCTTTGTGAGAGGATCCAACGGATCA
ACAGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR202855 protein sequence
Red=Cloning site Green=Tags(s)

MFIWTSGRTSSSYRQDEKRNIYQKIRDHDLLDKRKTVTALKAGEDRAILLGLAMMVCSIMMYFLLGITLL
RSYMQSVWTEEAQCALLNVSITETFNCSFSCGPDCWKLSQYPCLQVYVNLTSSGERLLLYHTEETMKINQ
KCSYIPKCGNNFEESMSLVSVVMENFRRHQHFPCYSDPEGNQKSVILTKLYSSNVLFHSLFWPTCMMAGG
VAIVAMVKLTQYLSLLCERIQRINR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_028231
ORF Size 708 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_028231.2, NP_082507.1
RefSeq Size 2947 bp
RefSeq ORF 708 bp
Locus ID 72413
UniProt ID Q9CZM9
MW 27.1 kDa
Gene Summary Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Acts as a negative regulator that confers rapid and complete inactivation of KCNMA1 channel complex (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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