Sfrs10 (BC086795) Mouse Tagged ORF Clone
CAT#: MR202755
- TrueORF®
Sfrs10 (Myc-DDK-tagged) - Mouse splicing factor, arginine/serine-rich 10 (transformer 2 homolog, Drosophila) (cDNA clone MGC:102169 IMAGE:6828358)
ORF Plasmid: tGFP
"BC086795" in other vectors (3)
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CNY 2,400.00
CNY 6,365.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | D16Ertd266e, SIG-41, TRA2B, TRA2beta |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR202755 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGACAGCGGCGAGCAGAACTACGGCGAGCGGGAATCCCGTTCTGCTTCCCGAAGTGGAAGTGCTC ACGGATCGGGGAAATCTGCACGGCATACACCTGCAAGGTCTCGCTCCAAGGAAGACTCAAGGCGTTCTAG ATCAAAGTCCAGGTCCAGGTCTGAATCTAGGTCTAGATCCAGAAGAAGTTCTAGAAGGCATTATACAAGG TCACGATCACGATCTCGCTCGCATAGACGATCCCGGAGCAGGTCTTACAGCCGAGATTATCGCAGGCGCC ACAGCCACAGCCATTCTCCCATGTCTACTCGAAGGCGTCATGTTGGGAACCGGGCAAATCCTGACCCCAA CTGTTGTCTTGGCGTGTTTGGGTTGAGCTTATACACCACAGAAAGAGACCTAAGAGAAGTGTTCTCTAAA TATGGCCCCATTGCTGATGTGTCTATTGTATATGACCAGCAATCTAGACGTTCAAGAGGATTTGCCTTTG TATATTTTGAAAATGTAGACGATGCCAAGGAAGCTAAAGAACGTGCCAATGGAATGGAGCTTGATGGGCG TCGAATTAGAGTCGATTTCTCTATAACAAAAAGGCCCCATACCCCAACACCAGGAATTTATATGGGGAGA CCCACTTATGGCAGTTCTCGCCGCCGAGACTATTATGACAGAGGGTACGATCGGGGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202755 protein sequence
Red=Cloning site Green=Tags(s) MSDSGEQNYGERESRSASRSGSAHGSGKSARHTPARSRSKEDSRRSRSKSRSRSESRSRSRRSSRRHYTR SRSRSRSHRRSRSRSYSRDYRRRHSHSHSPMSTRRRHVGNRANPDPNCCLGVFGLSLYTTERDLREVFSK YGPIADVSIVYDQQSRRSRGFAFVYFENVDDAKEAKERANGMELDGRRIRVDFSITKRPHTPTPGIYMGR PTYGSSRRRDYYDRGYDRG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | BC086795 |
ORF Size | 687 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC086795, AAH86795 |
RefSeq Size | 2012 bp |
RefSeq ORF | 689 bp |
Locus ID | 20462 |
MW | 26.7 kDa |
Gene Summary | Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing. Can either activate or suppress exon inclusion. Acts additively with RBMX to promote exon 7 inclusion of the survival motor neuron SMN2. Activates the splicing of MAPT/Tau exon 10. Alters pre-mRNA splicing patterns by antagonizing the effects of splicing regulators, like RBMX. Binds to the AG-rich SE2 domain in the SMN exon 7 RNA. Binds to pre-mRNA (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG202755 | Sfrs10 (tGFP-tagged) - Mouse splicing factor, arginine/serine-rich 10 (transformer 2 homolog, Drosophila) (cDNA clone MGC:102169 IMAGE:6828358) |
CNY 2,850.00 |
|
MR202755L3 | Lenti ORF clone of Sfrs10 (Myc-DDK-tagged) - Mouse splicing factor, arginine/serine-rich 10 (transformer 2 homolog, Drosophila) (cDNA clone MGC:102169 IMAGE:6828358) |
CNY 4,750.00 |
|
MR202755L4 | Lenti ORF clone of Sfrs10 (mGFP-tagged) - Mouse splicing factor, arginine/serine-rich 10 (transformer 2 homolog, Drosophila) (cDNA clone MGC:102169 IMAGE:6828358) |
CNY 4,750.00 |