United States
Germany
Japan
United Kingdom
China
Eif4h (BC014796) Mouse Tagged Lenti ORF Clone
CAT＃: MR202724L4
- LentiORF®
Lenti ORF clone of Eif4h (mGFP-tagged) - Mouse eukaryotic translation initiation factor 4H (cDNA clone MGC:11689 IMAGE:3962104)
"BC014796" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 4,750.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | Wscr1, mKIAA0038, Ef4h |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR202724).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC014796 |
| ORF Size | 684 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC014796, AAH14796 |
| RefSeq Size | 2374 bp |
| RefSeq ORF | 686 bp |
| Locus ID | 22384 |
| Gene Summary | This gene encodes eukaryotic translation initiation factor 4H (eIF4H) that plays a critical role in the process of protein synthesis. The encoded protein is an RNA-binding protein that, in concert with other translation initiation factors, helps unwind the 5' cap-proximal region of mRNA to prepare it for ribosomal attachment. Mice lacking the encoded protein displayed growth retardation with a significant reduction of body weight, a smaller brain volume and altered brain morphology. Behaviorally, mice lacking the encoded protein exhibit severe impairments of fear-related associative learning and memory formation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217765 | Eif4h (untagged) - Mouse eukaryotic translation initiation factor 4H (cDNA clone MGC:11689 IMAGE:3962104), (10ug) |
CNY 6,940.00 |
|
| MG202724 | Eif4h (tGFP-tagged) - Mouse eukaryotic translation initiation factor 4H (cDNA clone MGC:11689 IMAGE:3962104) |
CNY 2,850.00 |
|
| MR202724 | Eif4h (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4H (cDNA clone MGC:11689 IMAGE:3962104) |
CNY 2,400.00 |
|
| MR202724L3 | Lenti ORF clone of Eif4h (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4H (cDNA clone MGC:11689 IMAGE:3962104) |
CNY 4,750.00 |
