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Metapl1 (BC051534) Mouse Tagged ORF Clone
CAT＃: MR202412
- TrueORF®
Metapl1 (Myc-DDK-tagged) - Mouse methionine aminopeptidase-like 1 (cDNA clone MGC:63117 IMAGE:3466745)
ORF Plasmid: tGFP
"BC051534" in other vectors (4)
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CNY 2,400.00
CNY 2,945.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2310066F24Rik; 3110033D18Rik; AV117938; MAP 1D; Map1d; MetAP 1D; Metapl1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR202412 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAACTGAAGAGATAGATGCGCTTGTTCATTGGGAAATCATCAGGCATGACGCATATCCCTCACCTC TGGGCTATGGACGCTTTCCAAAATCTGTTTGCACCTCTGTAAACAACGTGCTCTGTCATGGCATTCCTGA CAGCCGACCTCTTCAGGATGGAGATATTATCAACATTGATGTCACGGTCTATTACAATGGCTACCATGGA GACACCTCTGAAACCTTTCTGGTGGGCAATGTGGATGAAAGTGGTAAAAAGTTAGTGGAGGTTGCCAGGA GGTGTAGAGATGAAGCGATTGCAGCCTGCAGAGCTGGGGCCCCCTTCTCTGTAATTGGAAACACAATCAG CCGCATAACTCATCAGAATGGTTTGCAAGTCTGTCCACATTTTGTGGGACATGGGATAGGATCATACTTT CATGGACACCCAGAAATTTGGCATCATGCAAATGACAATGATCTGCCCATGGAGGAGGGCATGGCTTTCA CCATAGAGCCAATCATCACTGAAGGTTCCCCAGAATTTAAAGTCCTTGAGGACGCATGGACCGTGGTCTC CCTAGACAACCAAAGGTCTGCGCAGTTCGAGCACACAGTCCTCATCACGCCAAGGGGAGTGGAGATACTG ACCAAACTGCCACAAGAAGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202412 protein sequence
Red=Cloning site Green=Tags(s) MTTEEIDALVHWEIIRHDAYPSPLGYGRFPKSVCTSVNNVLCHGIPDSRPLQDGDIINIDVTVYYNGYHG DTSETFLVGNVDESGKKLVEVARRCRDEAIAACRAGAPFSVIGNTISRITHQNGLQVCPHFVGHGIGSYF HGHPEIWHHANDNDLPMEEGMAFTIEPIITEGSPEFKVLEDAWTVVSLDNQRSAQFEHTVLITPRGVEIL TKLPQEA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC051534 |
| ORF Size | 651 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC051534, AAH51534 |
| RefSeq Size | 1131 bp |
| RefSeq ORF | 653 bp |
| Locus ID | 66559 |
| MW | 24.1 kDa |
| Gene Summary | Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC207093 | Metapl1 (untagged) - Mouse methionine aminopeptidase-like 1 (cDNA clone MGC:63117 IMAGE:3466745), (10ug) |
CNY 3,230.00 |
|
| MG202412 | Metapl1 (tGFP-tagged) - Mouse methionine aminopeptidase-like 1 (cDNA clone MGC:63117 IMAGE:3466745) |
CNY 2,850.00 |
|
| MR202412L3 | Lenti ORF clone of Metapl1 (Myc-DDK-tagged) - Mouse methionine aminopeptidase-like 1 (cDNA clone MGC:63117 IMAGE:3466745) |
CNY 4,750.00 |
|
| MR202412L4 | Lenti ORF clone of Metapl1 (mGFP-tagged) - Mouse methionine aminopeptidase-like 1 (cDNA clone MGC:63117 IMAGE:3466745) |
CNY 4,750.00 |
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