Rab17 (NM_008998) Mouse Tagged Lenti ORF Clone
CAT#: MR202342L3
- LentiORF®
Lenti ORF clone of Rab17 (Myc-DDK-tagged) - Mouse RAB17, member RAS oncogene family (Rab17), transcript variant 1
"NM_008998" in other vectors (5)
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CNY 4,750.00
CNY 600.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AW413472 |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR202342).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_008998 |
ORF Size | 645 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_008998.2, NP_033024.1 |
RefSeq Size | 1825 bp |
RefSeq ORF | 645 bp |
Locus ID | 19329 |
UniProt ID | P35292 |
Gene Summary | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in transcytosis, the directed movement of endocytosed material through the cell and its exocytosis from the plasma membrane at the opposite side. Mainly observed in epithelial cells, transcytosis mediates for instance, the transcellular transport of immunoglobulins from the basolateral surface to the apical surface. Most probably controls membrane trafficking through apical recycling endosomes in a post-endocytic step of transcytosis. Required for melanosome transport and release from melanocytes, it also regulates dendrite and dendritic spine development. May also play a role in cell migration.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC204653 | Rab17 (untagged) - Mouse RAB17, member RAS oncogene family (Rab17), transcript variant 1, (10ug) |
CNY 2,400.00 |
|
MG202342 | Rab17 (tGFP-tagged) - Mouse RAB17, member RAS oncogene family (Rab17) |
CNY 4,000.00 |
|
MR202342 | Rab17 (Myc-DDK-tagged) - Mouse RAB17, member RAS oncogene family (Rab17), transcript variant 1 |
CNY 2,400.00 |
|
MR202342L4 | Lenti ORF clone of Rab17 (mGFP-tagged) - Mouse RAB17, member RAS oncogene family (Rab17), transcript variant 1 |
CNY 4,750.00 |
|
MR202349 | Rab17 (Myc-DDK-tagged) - Mouse RAB17, member RAS oncogene family (Rab17), transcript variant 1 |
CNY 1,416.00 |