Rhod (NM_007485) Mouse Tagged ORF Clone

CAT#: MR202278

  • TrueORF®

Rhod (Myc-DDK-tagged) - Mouse ras homolog gene family, member D (Rhod)

ORF Plasmid: DDK tGFP



  "NM_007485" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,081.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms AI326383; Arhd; Rho; RhoHP1; RhoM
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR202278 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACGCGTCCCAGGTTGCGGGAGAAGAGGCGCCGCAGAGCGGGCACTCGGTCAAGGTGGTCCTGGTGG
GCGACGGGGGCTGCGGGAAGACGTCACTGATGATGGTCTTCGCCAAAGGGGCCTTCCCAGAGAGCTACAG
TCCCACAGTGTTTGAGCGCTATAATGCCACTCTGCAGATGAAGGGTAAACCTGTGCACCTCCAAATCTGG
GACACAGCCGGGCAAGATGACTATGACCGCCTCCGGCCCTTGTTCTATCCTGATGCCAATGTCTTGCTCC
TCTGCTTCGATGTGACCAATCCAAACAGCTTTGACAACGTCTCCAACCGGTGGTACCCAGAGGTGACACA
TTTCTGCAAGGGAGTGCCCATCATTGTTGTGGGCTGCAAGATAGACCTGCGTAAGGACAAGGTGCTGGTG
AACAACCTGCGGAAGAAAAGACTGGAGCCCGTGACCTACCACAGGGGCCACGATATGGCAAGGTCTGTGG
GAGCGGTGGCCTATCTTGAGTGTTCAGCTCGGCTCCATGACAACGTGGAAGCCGTCTTCCAGGAAGCAGC
AGAAGTGGCTCTCAGCAGTCGCAGACATAACTTTTGGCGGCGGATTACTCAGAATTGTTGCTTGGCCACC


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>MR202278 protein sequence
Red=Cloning site Green=Tags(s)

MNASQVAGEEAPQSGHSVKVVLVGDGGCGKTSLMMVFAKGAFPESYSPTVFERYNATLQMKGKPVHLQIW
DTAGQDDYDRLRPLFYPDANVLLLCFDVTNPNSFDNVSNRWYPEVTHFCKGVPIIVVGCKIDLRKDKVLV
NNLRKKRLEPVTYHRGHDMARSVGAVAYLECSARLHDNVEAVFQEAAEVALSSRRHNFWRRITQNCCLAT

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_007485
ORF Size 633 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_007485.5
RefSeq Size 1338 bp
RefSeq ORF 633 bp
Locus ID 11854
UniProt ID P97348
MW 23.6 kDa
Gene Summary Involved in endosome dynamics. May coordinate membrane transport with the function of the cytoskeleton. Involved in the internalization and trafficking of activated tyrosine kinase receptors such as PDGFRB. Participates in the reorganization of actin cytoskeleton; the function seems to involve WHAMM and includes regulation of filopodia formation and actin filament bundling. Can modulate the effect of DAPK3 in reorganization of actin cytoskeleton and focal adhesion dissolution.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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