Guca1b (NM_146079) Mouse Tagged ORF Clone
CAT#: MR202065
- TrueORF®
Guca1b (Myc-DDK-tagged) - Mouse guanylate cyclase activator 1B (Guca1b)
ORF Plasmid: tGFP
"NM_146079" in other vectors (4)
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CNY 2,400.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | BC018258; GCAP2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR202065 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCAGCAGCTGAGCTGGGAAGAGGCGGAGGCGGCCGGGGAGATGGATGTGGCGGAGTTGCAGGAGT GGTACAAGAAGTTTGTCGTGGAGTGTCCCAGTGGGACGCTCTTCATGCACGAGTTCAAGCGCTTCTTCAA GGTCACGGGCAATGAGGAGGCCAGCCAGTATGTAGAGAGCATGTTCCGAGCCTTCGACAAGAATGGGGAC AACACCATTGACTTCCTGGAGTATGTGGCAGCCCTGAACCTCGTGCTGAGGGGCAGCCTGGAACACAAGC TCAAGTGGACCTTCAAGATCTATGACAAGGACCGAAATGGCTGCATCGACCGCCTGGAACTCCTGGACAT TGTGGAGGCGATTTACAAGCTGAAGAAAGCCTGCCGAGCGGAGCTGGATCTGGAACACCAGGGCCAGCTG CTCACGCCCGAGGAGGTGGTGGACAGGATCTTCCTCCTGGTGGACGAGAATGGAGACGGCCAGCTGTCTC TGACCGAGTTCATCGAGGGCGCCCGTCGCGATAAGTGGGTAATGAAGATGCTACAAATGGATATCAACCC AGGCTGCTGGATCACGCAGCAGAGGCGGAGAAGCGCCATGTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202065 protein sequence
Red=Cloning site Green=Tags(s) MGQQLSWEEAEAAGEMDVAELQEWYKKFVVECPSGTLFMHEFKRFFKVTGNEEASQYVESMFRAFDKNGD NTIDFLEYVAALNLVLRGSLEHKLKWTFKIYDKDRNGCIDRLELLDIVEAIYKLKKACRAELDLEHQGQL LTPEEVVDRIFLLVDENGDGQLSLTEFIEGARRDKWVMKMLQMDINPGCWITQQRRRSAMF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_146079 |
ORF Size | 606 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_146079.1, NP_666191.1 |
RefSeq Size | 1681 bp |
RefSeq ORF | 606 bp |
Locus ID | 107477 |
UniProt ID | Q8VBV8 |
MW | 23.5 kDa |
Gene Summary | Stimulates guanylyl cyclase 1 (GC1) and GC2 when free calcium ions concentration is low, and GC1 and GC2 when free calcium ions concentration is elevated. This Ca(2+)-sensitive regulation of GC is a key event in recovery of the dark state of rod photoreceptors following light exposure (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Impaired Association of Retinal Degeneration-3 with Guanylate Cyclase-1 and Guanylate Cyclase Activating Protein-1 Leads to Leber Congenital Amaurosis-1
,Zulliger, R;Naash, MI;Rajala, RV;Molday, RS;Azadi, S;,
J. Biol. Chem.
,PubMed ID 25477517
[GUCA1B]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC201675 | Guca1b (untagged) - Mouse guanylate cyclase activator 1B (Guca1b), (10ug) |
CNY 2,400.00 |
|
MG202065 | Guca1b (tGFP-tagged) - Mouse guanylate cyclase activator 1B (Guca1b) |
CNY 2,850.00 |
|
MR202065L3 | Lenti ORF clone of Guca1b (Myc-DDK-tagged) - Mouse guanylate cyclase activator 1B (Guca1b) |
CNY 4,750.00 |
|
MR202065L4 | Lenti ORF clone of Guca1b (mGFP-tagged) - Mouse guanylate cyclase activator 1B (Guca1b) |
CNY 4,750.00 |