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Nfu1 (NM_020045) Mouse Tagged ORF Clone
CAT＃: MR201993
- TrueORF®
Nfu1 (Myc-DDK-tagged) - Mouse NFU1 iron-sulfur cluster scaffold homolog (S. cerevisiae) (Nfu1), nuclear gene encoding mitochondrial protein, transcript variant 2
ORF Plasmid: tGFP
"NM_020045" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 0610006G17Rik; CGI-33; Hirip5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR201993 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTATTCAAACACAAGACACCCCAAATCCCAACAGCTTAAAGTTTATACCAGGAAAGCCGGTTCTTG AGACGAGGACCATGGATTTCCCCACCCCTGCTGCAGCTTTCCGCTCCCCTCTGGCCAGGCAGTTATTCAG AATTGAAGGTGTGAAAAGCGTCTTCTTCGGACCAGATTTCATCACAGTCACAAAGGAGAATGAAGAGTTA GACTGGAATTTACTGAAACCAGACATTTATGCCACAATTATGGACTTCTTTGCATCTGGCTTACCCCTAG TGACCGAGGAGACGCCTCCGCCTCCGGGAGAAGCAGGCTCTGAGGAAGATGACGAAGTCGTGGCAATGAT TAAAGAACTGCTAGATACTAGAATAAGGCCAACTGTGCAGGAGGATGGGGGCGACGTGATCTACAGGGGC TTTGAAGATGGCATCGTGCGGCTGAAGCTCCAGGGCTCCTGCACCAGCTGCCCCAGCTCCATCATCACCC TGAAGAGCGGGATTCAGAACATGCTGCAGTTTTATATTCCAGAAGTGGAAGGTGTGGAGCAGGTGATGGA TGATGACGAATCAGATGAAAAAGAAGCAAATTCATCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201993 protein sequence
Red=Cloning site Green=Tags(s) MFIQTQDTPNPNSLKFIPGKPVLETRTMDFPTPAAAFRSPLARQLFRIEGVKSVFFGPDFITVTKENEEL DWNLLKPDIYATIMDFFASGLPLVTEETPPPPGEAGSEEDDEVVAMIKELLDTRIRPTVQEDGGDVIYRG FEDGIVRLKLQGSCTSCPSSIITLKSGIQNMLQFYIPEVEGVEQVMDDDESDEKEANSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020045 |
| ORF Size | 600 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020045.2, NP_064429.1 |
| RefSeq Size | 965 bp |
| RefSeq ORF | 768 bp |
| Locus ID | 56748 |
| UniProt ID | Q9QZ23 |
| MW | 22.1 kDa |
| Gene Summary | Iron-sulfur cluster scaffold protein which can assemble [4Fe-4S] clusters and deliver them to target proteins.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Assembly of the [4Fe-4S] cluster of NFU1 requires the coordinated donation of two [2Fe-2S] clusters from the scaffold proteins, ISCU2 and ISCA1
,Jain, A;Singh, A;Maio, N;Rouault, TA;,
Hum. Mol. Genet.
,PubMed ID 32776106
[NFU1]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203723 | Nfu1 (untagged) - Mouse NFU1 iron-sulfur cluster scaffold homolog (S. cerevisiae) (Nfu1), nuclear gene encoding mitochondrial protein, transcript variant 2, (10ug) |
CNY 2,400.00 |
|
| MG201993 | Nfu1 (tGFP-tagged) - Mouse NFU1 iron-sulfur cluster scaffold homolog (S |
CNY 2,850.00 |
|
| MR201993L3 | Lenti ORF clone of Nfu1 (Myc-DDK-tagged) - Mouse NFU1 iron-sulfur cluster scaffold homolog (S. cerevisiae) (Nfu1), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 4,750.00 |
|
| MR201993L4 | Lenti ORF clone of Nfu1 (mGFP-tagged) - Mouse NFU1 iron-sulfur cluster scaffold homolog (S. cerevisiae) (Nfu1), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 4,750.00 |
