Commd9 (NM_029635) Mouse Tagged ORF Clone

CAT#: MR201989

  • TrueORF®

Commd9 (Myc-DDK-tagged) - Mouse COMM domain containing 9 (Commd9)

ORF Plasmid: DDK tGFP



  "NM_029635" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,400.00

CNY 3,705.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 1810029F08Rik; AA409293
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR201989 representing NM_029635
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCCTTGACAGCAGAGCATTTCGTGGCGCTCCAGAGCCTACTGAAGGCCTCCTCAAAAGATGTTG
TCCGACAACTGTGCCAAGAAAGCTTCTCCAGTTCATGCCTTGACTCAGAAAGCCTCCTAGATAAGACGTG
CTCCAGCTTGTCTGTGCCCCAGGGGGAGGCAGCGCAACTGCTGCAGGCTCTGCACCACTTCACCAGGCTG
GTGGCCTTCCGTGACCTGTCCTCTGCTGAGGCGATTCTGGCTCTCTTTCCGGAAAATTTCCACCAAAACC
TCAAAAACCTGCTGACAAAAATCATCGTGGAGCACATATCGACTTGGAGAGCTGAAGCCCAAGCAAACCA
GATCTCTCTGCCACGCCTGGTGGACCTGGACTGGAGAGTGGACATCAAAACCTCCTCTGACAACATCAGC
CGCATGGCTGTCCCCACCTGCCTGCTCCAGATGAAGATCCAGGAGGATCCTAGTCTGTGTGGAGAGAAAC
CCTCCATCTCGGCTGTGACCATGGAGCTGAGCAAAGAGACGCTGGACACCATGTTAGATGGGCTGGGTCG
CATTCGGGACCAGCTTTCTGCTGTAGCTAATAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR201989 representing NM_029635
Red=Cloning site Green=Tags(s)

MAALTAEHFVALQSLLKASSKDVVRQLCQESFSSSCLDSESLLDKTCSSLSVPQGEAAQLLQALHHFTRL
VAFRDLSSAEAILALFPENFHQNLKNLLTKIIVEHISTWRAEAQANQISLPRLVDLDWRVDIKTSSDNIS
RMAVPTCLLQMKIQEDPSLCGEKPSISAVTMELSKETLDTMLDGLGRIRDQLSAVANK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_029635
ORF Size 594 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_029635.3, NP_083911.1
RefSeq Size 1175 bp
RefSeq ORF 597 bp
Locus ID 76501
UniProt ID Q8K2Q0
MW 21.9 kDa
Gene Summary May modulate activity of cullin-RING E3 ubiquitin ligase (CRL) complexes. May down-regulate activation of NF-kappa-B. Modulates Na(+) transport in epithelial cells by regulation of apical cell surface expression of amiloride-sensitive sodium channel (ENaC) subunits.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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