Msi2h (BC109368) Mouse Tagged ORF Clone

CAT#: MR201867

  • TrueORF®

Msi2h (Myc-DDK-tagged) - Mouse Musashi homolog 2 (Drosophila) (cDNA clone MGC:118040 IMAGE:5249862)

ORF Plasmid: DDK tGFP



  "BC109368" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,400.00

CNY 2,945.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms msi2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR201867 representing BC109368
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCAAATGGGAGCCCAGGCACCTCGGGCAGCGCCAACGACTCCCAGCACGACCCCGGTAAAATGT
TTATCGGTGGACTGAGCTGGCAGACCTCACCAGATAGCCTTAGAGACTATTTTAGCAAATTTGGAGAAAT
TAGAGAATGTATGGTCATGAGAGATCCCACAACGAAACGCTCCAGAGGCTTCGGTTTCGTCACCTTCGCA
GACCCAGCAAGTGTAGATAAAGTATTAGGTCAGCCCCACCATGAGTTAGATTCCAAGACGATTGACCCAA
AAGTTGCATTTCCTCGTCGAGCGCAACCTAAGAGTCAAGAGCAGGGAGGCTCCTGGAAACGCACATGGAC
CACAGTCCAAGCTCGGGCTGCCTCAGGTGACCAGAAAAAGGTTGGCTTGTCAGACTCTGCTGCCCTCTTC
CCGGCTCTTCCCGTCCCTCCGCGGCCTGGCAGACGAGGCTTTAGAGGCTTTCTGATGGGCAACCCGGAGC
TTTCTGCAATCCAGCTCTGGCTTTTCTTACCCCAGCCGTTCTTTCTCACAGCGAAGAGGATACAATTCAG
CACCAATAATGCCGAGCAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR201867 representing BC109368
Red=Cloning site Green=Tags(s)

MEANGSPGTSGSANDSQHDPGKMFIGGLSWQTSPDSLRDYFSKFGEIRECMVMRDPTTKRSRGFGFVTFA
DPASVDKVLGQPHHELDSKTIDPKVAFPRRAQPKSQEQGGSWKRTWTTVQARAASGDQKKVGLSDSAALF
PALPVPPRPGRRGFRGFLMGNPELSAIQLWLFLPQPFFLTAKRIQFSTNNAEQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC109368
ORF Size 579 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC109368, AAI09369
RefSeq Size 711 bp
RefSeq ORF 581 bp
Locus ID 76626
MW 26.1 kDa
Gene Summary RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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