Nkiras2 (NM_028024) Mouse Tagged ORF Clone
CAT#: MR201833
- TrueORF®
Nkiras2 (Myc-DDK-tagged) - Mouse NFKB inhibitor interacting Ras-like protein 2 (Nkiras2)
ORF Plasmid: tGFP
"NM_028024" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2410003M04Rik; 4930527H08Rik; D630018G21Rik; KBRAS2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR201833 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAGAGCTGCAAGGTGGTCGTGTGCGGCCAGGCATCTGTGGGCAAAACTTCAATCCTTGAGCAGC TTCTGTACGGGAACCATGTCGTGGGTTCTGAGATGATCGAGACCCAGGAGGACATCTATGTAGGCTCCAT CGAGACAGACCGAGGGGTGCGGGAGCAGGTGCGTTTCTATGATACACGGGGTCTCCGGGATGGGGCCGAG CTGCCCAAGCACTGCTTCTCCTGCACCGATGGCTACGTCCTGGTCTACAGCACAGACAGCCGAGAGTCGT TCCAGCGCGTCGAGCTGCTCAAGAAGGAGATCGACAAGTCCAAGGACAAGAAGGAGGTCACCATCGTGGT CCTTGGCAACAAGTGTGACCTGCAGGAGCAGCGTCGCGTAGACCCTGACGTGGCCCAGCACTGGGCCAAG TCCGAGAAGGTGAAGCTATGGGAGGTGTCGGTGGCTGACCGACGCTCCCTCCTAGAGCCTTTCATCTACC TGGCCAGCAAGATGACCCAGCCGCAGAGCAAGTCTGCCTTCCCCCTCAGCCGCAAGAACAAGGGCAGCGG CTCCTTGGATGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201833 protein sequence
Red=Cloning site Green=Tags(s) MGKSCKVVVCGQASVGKTSILEQLLYGNHVVGSEMIETQEDIYVGSIETDRGVREQVRFYDTRGLRDGAE LPKHCFSCTDGYVLVYSTDSRESFQRVELLKKEIDKSKDKKEVTIVVLGNKCDLQEQRRVDPDVAQHWAK SEKVKLWEVSVADRRSLLEPFIYLASKMTQPQSKSAFPLSRKNKGSGSLDG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_028024 |
ORF Size | 576 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_028024.2, NP_082300.1 |
RefSeq Size | 2013 bp |
RefSeq ORF | 576 bp |
Locus ID | 71966 |
UniProt ID | Q9CR56 |
MW | 21.5 kDa |
Gene Summary | Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and nuclear localization of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC201071 | Nkiras2 (untagged) - Mouse NFKB inhibitor interacting Ras-like protein 2 (Nkiras2), (10ug) |
CNY 2,400.00 |
|
MG201833 | Nkiras2 (tGFP-tagged) - Mouse NFKB inhibitor interacting Ras-like protein 2 (Nkiras2) |
CNY 2,850.00 |
|
MR201833L3 | Lenti ORF clone of Nkiras2 (Myc-DDK-tagged) - Mouse NFKB inhibitor interacting Ras-like protein 2 (Nkiras2) |
CNY 4,750.00 |
|
MR201833L4 | Lenti ORF clone of Nkiras2 (mGFP-tagged) - Mouse NFKB inhibitor interacting Ras-like protein 2 (Nkiras2) |
CNY 4,750.00 |
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