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Fgl1 (BC029734) Mouse Tagged ORF Clone
CAT＃: MR201613
- TrueORF®
Fgl1 (Myc-DDK-tagged) - Mouse fibrinogen-like protein 1 (cDNA clone MGC:25810 IMAGE:4158843)
ORF Plasmid: tGFP
"BC029734" in other vectors (3)
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CNY 1,900.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Mfire1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR201613 representing BC029734
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAAAGATTTACAGCTTCGTCCTGGTCGCCATTGCTCTGATGATGGGAAGGGAAGGTTGGGCCCTCG AGAGTGAGAGCTGCTTGCGGGAGCAGGTAAGGCTCAGGGCTCAGGTGCACCAGCTTGAGACCCGGGTCAA ACAACAACAGACCATGATTGCACAGCTCTTGCATGAGAAGGAAGTCCAGTTTCTGGATAAAGGATCGGAG AACAGTTTCATTGACCTTGGAGGCAAGAGGCAGTATGCAGATTGTTCAGAGATTTACAATGACGGATTTA AGCAGAGTGGATTTTACAAAATCAAACCTCTTCAGAGCCTGGCAGAATTCTCTGTTTATTGTGACATGTC TGATGGAGGGGGATGGACTGTAATTCAGAGACGATCTGATGGCAGTGAGAACTTTAACAGGGGTTGGAAT GACTATGAAAATGGCTTTGGAAACTTTGTCCAAAACAATGGCGAATACTGGCTGGGTAACAAAAACATTA ACTTGCTAACTATTCAAGGAGACTACACTTTAAATCGACCTGACAGATTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201613 representing BC029734
Red=Cloning site Green=Tags(s) MGKIYSFVLVAIALMMGREGWALESESCLREQVRLRAQVHQLETRVKQQQTMIAQLLHEKEVQFLDKGSE NSFIDLGGKRQYADCSEIYNDGFKQSGFYKIKPLQSLAEFSVYCDMSDGGGWTVIQRRSDGSENFNRGWN DYENGFGNFVQNNGEYWLGNKNINLLTIQGDYTLNRPDRF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC029734 |
| ORF Size | 540 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC029734.1 |
| RefSeq Size | 1104 bp |
| RefSeq ORF | 542 bp |
| Locus ID | 234199 |
| MW | 40.5 kDa |
| Gene Summary | Immune suppressive molecule that inhibits antigen-specific T-cell activation by acting as a major ligand of LAG3 (PubMed:30580966). Responsible for LAG3 T-cell inhibitory function (PubMed:30580966). Binds LAG3 independently from MHC class II (MHC-II) (PubMed:30580966). In addition to its role in inhibiting inflammatory immune responses, also plays a role in hepatocytes (By similarity). Under normal physiological conditions, primarily secreted from hepatocytes and contributes to its mitogenic and metabolic functions (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG201613 | Fgl1 (tGFP-tagged) - Mouse fibrinogen-like protein 1 (cDNA clone MGC:25810 IMAGE:4158843) |
CNY 2,090.00 |
|
| MR201613L3 | Lenti ORF clone of Fgl1 (Myc-DDK-tagged) - Mouse fibrinogen-like protein 1 (cDNA clone MGC:25810 IMAGE:4158843) |
CNY 3,800.00 |
|
| MR201613L4 | Lenti ORF clone of Fgl1 (mGFP-tagged) - Mouse fibrinogen-like protein 1 (cDNA clone MGC:25810 IMAGE:4158843) |
CNY 3,800.00 |
