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D2Wsu81e (BC078441) Mouse Tagged ORF Clone
CAT＃: MR201597
- TrueORF®
D2Wsu81e (Myc-DDK-tagged) - Mouse DNA segment, Chr 2, Wayne State University 81, expressed (cDNA clone MGC:90552 IMAGE:5686308)
ORF Plasmid: tGFP
"BC078441" in other vectors (4)
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CNY 2,400.00
CNY 2,945.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 9930028P05; AL033358 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR201597 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAAACTGGAGCGGCAGCGTGCCCAAGAGGAAGAGGCTAAACGCCAGGAAGAGGAGGAAGAGGCGG CGGCCCAGAGGAGCAACCAAGGACGGCCTTACACTCTGAGTGTGGCCCTGCCAGGCTCCATCCTGGACAA TGCCCAGTCACCTGAGCTCCGCACCTACCTGGCTGGCCAGATCGCCAGAGCCTGCACCATCTTCTGTGTG GATGAGATTGTGGTGTTCGATGAGGAGGGCCAAGATACCAAGAGTGTGGAAGGAGAATTCAGGGGAGTTG GCAAGAAAGGGCAGGCATGTGTCCAGCTGGCCCGCATCCTACAGTACCTGGAGTGTCCACAGTACCTGAG AAAGGCGTTCTTCCCCAAGCACCAAGATCTGCAGTTTGCAGGAATCCTCAACCCCTTGGACAGCCCTCAC CACATGCGTCAGGACGAAGAGTCTGAGTTTCGAGAAGGCGTCGTGGTGGACCGGCCCACCAAGGCAGGCC ATGGCTCTTTGGTCAACTGTGGAATGAAGAAGCTTCTCTTCAGGAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201597 protein sequence
Red=Cloning site Green=Tags(s) MKKLERQRAQEEEAKRQEEEEEAAAQRSNQGRPYTLSVALPGSILDNAQSPELRTYLAGQIARACTIFCV DEIVVFDEEGQDTKSVEGEFRGVGKKGQACVQLARILQYLECPQYLRKAFFPKHQDLQFAGILNPLDSPH HMRQDEESEFREGVVVDRPTKAGHGSLVNCGMKKLLFRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC078441 |
| ORF Size | 537 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC078441, AAH78441 |
| RefSeq Size | 2440 bp |
| RefSeq ORF | 539 bp |
| Locus ID | 227695 |
| MW | 20.3 kDa |
| Gene Summary | Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase. Also involved in chromosome alignment. May promote centrosome maturation probably by recruiting A-kinase anchor protein AKAP9 to centrosomes in early mitosis. Binds specifically to miRNA MIR145 hairpin, regulates MIR145 expression at a postranscriptional level (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC206986 | D2Wsu81e (untagged) - Mouse DNA segment, Chr 2, Wayne State University 81, expressed (cDNA clone MGC:90552 IMAGE:5686308), (10ug) |
CNY 3,230.00 |
|
| MG201597 | D2Wsu81e (tGFP-tagged) - Mouse DNA segment, Chr 2, Wayne State University 81, expressed (cDNA clone MGC:90552 IMAGE:5686308) |
CNY 2,850.00 |
|
| MR201597L3 | Lenti ORF clone of D2Wsu81e (Myc-DDK-tagged) - Mouse DNA segment, Chr 2, Wayne State University 81, expressed (cDNA clone MGC:90552 IMAGE:5686308) |
CNY 4,750.00 |
|
| MR201597L4 | Lenti ORF clone of D2Wsu81e (mGFP-tagged) - Mouse DNA segment, Chr 2, Wayne State University 81, expressed (cDNA clone MGC:90552 IMAGE:5686308) |
CNY 4,750.00 |
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