Pthlh (NM_008970) Mouse Tagged ORF Clone
CAT#: MR201519
- TrueORF®
Pthlh (Myc-DDK-tagged) - Mouse parathyroid hormone-like peptide (Pthlh)
ORF Plasmid: tGFP
"NM_008970" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | PLP; Pt; PTH-l; PTH-like; Pthrp |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR201519 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCGGAGGCTGGTTCAGCAGTGGAGTGTCCTGGTATTCCTGCTCAGCTACTCCGTGCCCTCCCGCG GGCGTTCGGTGGAGGGGCTTGGCCGCAGGCTCAAACGCGCTGTGTCTGAACATCAGCTACTGCATGACAA GGGCAAGTCCATCCAAGACTTGCGCCGCCGTTTCTTCCTCCACCATCTGATCGCGGAGATCCACACAGCC GAAATCAGAGCTACCTCGGAGGTGTCCCCCAACTCCAAACCTGCTCCCAACACCAAAAACCACCCCGTGC GGTTTGGGTCAGACGATGAGGGCAGATACCTAACTCAGGAAACCAACAAGGTGGAGACGTACAAAGAACA GCCACTCAAGACACCCGGGAAGAAGAAGAAAGGCAAGCCTGGGAAACGCAGAGAACAGGAGAAAAAGAAG CGAAGGACTCGGTCTGCCTGGCCAAGCACAGCTGCGAGTGGCCTGCTTGAGGACCCCCTGCCCCACACCT CCAGGACCTCGCTGGAGCCCAGCTTAAGGACGCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201519 protein sequence
Red=Cloning site Green=Tags(s) MLRRLVQQWSVLVFLLSYSVPSRGRSVEGLGRRLKRAVSEHQLLHDKGKSIQDLRRRFFLHHLIAEIHTA EIRATSEVSPNSKPAPNTKNHPVRFGSDDEGRYLTQETNKVETYKEQPLKTPGKKKKGKPGKRREQEKKK RRTRSAWPSTAASGLLEDPLPHTSRTSLEPSLRTH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_008970 |
ORF Size | 528 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008970.1, NP_032996.1 |
RefSeq Size | 1512 bp |
RefSeq ORF | 528 bp |
Locus ID | 19227 |
MW | 20.1 kDa |
Gene Summary | This gene encodes a member of the parathyroid family of hormones that possesses distinct paracrine and intracrine signaling roles such as regulation of circulating calcium, transplacental calcium transport, osteoclast inhibition, renal bicarbonate excretion and regulation of apoptosis. The encoded protein undergoes proteolytic processing to generate multiple active peptides with distinct signaling functions. The homozygous deletion of this gene leads to death shortly after birth with a chondrodystrophic phenotype characterized by premature chondrocyte differentiation and accelerated bone formation. [provided by RefSeq, Jul 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC203245 | Pthlh (untagged) - Mouse parathyroid hormone-like peptide (Pthlh), (10ug) |
CNY 2,400.00 |
|
MG201519 | Pthlh (tGFP-tagged) - Mouse parathyroid hormone-like peptide (Pthlh) |
CNY 2,850.00 |
|
MR201519L3 | Lenti ORF clone of Pthlh (Myc-DDK-tagged) - Mouse parathyroid hormone-like peptide (Pthlh) |
CNY 4,800.00 |
|
MR201519L4 | Lenti ORF clone of Pthlh (mGFP-tagged) - Mouse parathyroid hormone-like peptide (Pthlh) |
CNY 4,750.00 |