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Tprkb (BC027162) Mouse Tagged ORF Clone
CAT＃: MR201518
- TrueORF®
Tprkb (Myc-DDK-tagged) - Mouse Tp53rk binding protein (cDNA clone MGC:37089 IMAGE:4951567)
ORF Plasmid: tGFP
"BC027162" in other vectors (3)
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CNY 2,400.00
CNY 2,945.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Cgi-121, mDRWMS1, DRWMS1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR201518 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCTATCACAGCAGCTGGACTTGTTTCCTGAATGCAGGGTGACCCTTCTGTTATTTAAAGATGTAA AAAATGCAGGAGACTTGAGAAAAAAGGCCATGGAAGGATCTATTGATGGTTCATTGATAAACCCCAATGT GATTGTCGATCCATTTCAGATACTTGTGGCAGCAAACAAAGCCGTTCATCTCCACAGACTGGGAAAAATG AAGACAAGAACTCTGTCTACTGAAATTATCTTCAACCTCTCCCCAAATAATAATATTTCAGAGGCCTTGA AGAAATTTGGCATTTCAGAAACTAACACTTCAGTTCTGATTGTTTACATTGAAGATGGAAGCAAACAAGT ACCACAGGAACACCTAGTATCTCAAGTGGAAGGCCAGCAGGTGCCTTTGGAAAGTCTTCCAGAAATAACA AGGCTCTCGGAAGTCAAGAAGATATATAAACTGTCATCACAAGAAGAGAGGATTGGGACGTTATTGGATG CTATCATTTGTAGAATGTCAACGAAGGATGTTTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201518 protein sequence
Red=Cloning site Green=Tags(s) MQLSQQLDLFPECRVTLLLFKDVKNAGDLRKKAMEGSIDGSLINPNVIVDPFQILVAANKAVHLHRLGKM KTRTLSTEIIFNLSPNNNISEALKKFGISETNTSVLIVYIEDGSKQVPQEHLVSQVEGQQVPLESLPEIT RLSEVKKIYKLSSQEERIGTLLDAIICRMSTKDVL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC027162 |
| ORF Size | 525 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC027162, AAH27162 |
| RefSeq Size | 980 bp |
| RefSeq ORF | 527 bp |
| Locus ID | 69786 |
| MW | 19.6 kDa |
| Gene Summary | Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. TPRKB acts as an allosteric effector that regulates the t(6)A activity of the complex. TPRKB is not required for tRNA modification.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG201518 | Tprkb (tGFP-tagged) - Mouse Tp53rk binding protein (cDNA clone MGC:37089 IMAGE:4951567) |
CNY 2,850.00 |
|
| MR201518L3 | Lenti ORF clone of Tprkb (Myc-DDK-tagged) - Mouse Tp53rk binding protein (cDNA clone MGC:37089 IMAGE:4951567) |
CNY 4,750.00 |
|
| MR201518L4 | Lenti ORF clone of Tprkb (mGFP-tagged) - Mouse Tp53rk binding protein (cDNA clone MGC:37089 IMAGE:4951567) |
CNY 4,750.00 |
