Bcas2 (BC023382) Mouse Tagged ORF Clone

CAT#: MR201239

  • TrueORF®

Bcas2 (Myc-DDK-tagged) - Mouse breast carcinoma amplified sequence 2 (cDNA clone MGC:31032 IMAGE:5151893)

ORF Plasmid: DDK tGFP



  "BC023382" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 1,200.00

CNY 2,945.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms MGC7712
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR201239 representing BC023382
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGAAATGAATTTGAAAGACTCGCTGCTCGACAACCGATTGAATTACTCAGCATGAAACGATATGAAC
TTCCAGCCCCTTCCTCAGGTCAAAAAAATGACATTACTGCATGGCAAGAATGTGTAAACAATTCTATGGC
TCAGTTGGAGCACCAGGCGGTCCGGATCGAGAATCTGGAGCTGATGTCACAGCATGGATGCAATGCCTGG
AAGGTGTACAATGAAAATCTTGTTCATATGATTGAACATGCACAGAAAGAGCTTCAGAAGTTAAGGAAAC
ATATTCAAGATTTGAACTGGCAGCGAAAGAACATGCAGCTTACAGCTGGATCTAAGCTGAGAGAAATGGA
GTCAAACTGGGTGTCGCTGGTGAGTAAGAACTATGAGATTGAGCGGACGATTGTCCAGCTGGAGAACGAG
ATCTATCAGATCAAGCAGCAGCACGGGGAGGCCAACAAGGAAAACATCCGCCAAGACTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR201239 representing BC023382
Red=Cloning site Green=Tags(s)

MRNEFERLAARQPIELLSMKRYELPAPSSGQKNDITAWQECVNNSMAQLEHQAVRIENLELMSQHGCNAW
KVYNENLVHMIEHAQKELQKLRKHIQDLNWQRKNMQLTAGSKLREMESNWVSLVSKNYEIERTIVQLENE
IYQIKQQHGEANKENIRQDF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC023382
ORF Size 480 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC023382.1
RefSeq Size 1407 bp
RefSeq ORF 482 bp
Locus ID 68183
MW 51.6 kDa
Gene Summary Required for pre-mRNA splicing as component of the activated spliceosome. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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