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Trfp (BC060122) Mouse Tagged ORF Clone
CAT＃: MR200965
- TrueORF®
Trfp (Myc-DDK-tagged) - Mouse Trf (TATA binding protein-related factor)-proximal protein homolog (Drosophila) (cDNA clone MGC:63299
ORF Plasmid: tGFP
"BC060122" in other vectors (4)
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CNY 1,200.00
CNY 2,945.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110011O05Rik; 2410115I17Rik; AU018348; Trfp |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR200965 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGTGACGTGTGTGTCCCAGATGCCTGTGGCTGAGGGCAAGAGTCTCCAGCAGACAGTGGAGCTCC TCACCAAGAAACTGGAGATGCTTGGAGCAGAGAAACAAGGGACATTTTGTGTGGACTGTGAGACTTACCA CACAGCTGCCTCTACCCTGGGCAGCCAAGGCCAGGCTGGGAAGCTGATGTACGTGATGCATAACTCTGAG TACCCACTGAGCTGCTTTGCCCTCTTTGAGAATGGCCCTTGCCTCATTGCTGACACCAACTTCGATGTGC TTATGGTGAAGCTCAAGGGCTTCTTCCAGAGTGCCAAGGCCAGCAAGATTGAGACCCGGGGCACTCGCTA CCAGTATTGTGACTTCCTGGTGAAGGTGGGCACGGTCACAATGGGGCCCAGTGCCCGTGGCATCTCTGTG GAGGTAAGACCTTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR200965 protein sequence
Red=Cloning site Green=Tags(s) MGVTCVSQMPVAEGKSLQQTVELLTKKLEMLGAEKQGTFCVDCETYHTAASTLGSQGQAGKLMYVMHNSE YPLSCFALFENGPCLIADTNFDVLMVKLKGFFQSAKASKIETRGTRYQYCDFLVKVGTVTMGPSARGISV EVRPW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC060122 |
| ORF Size | 435 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC060122, AAH60122 |
| RefSeq Size | 5774 bp |
| RefSeq ORF | 437 bp |
| Locus ID | 56771 |
| MW | 15.9 kDa |
| Gene Summary | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC206881 | Trfp (untagged) - Mouse Trf (TATA binding protein-related factor)-proximal protein homolog (Drosophila) (cDNA clone MGC:63299, (10ug) |
CNY 3,230.00 |
|
| MG200965 | Trfp (tGFP-tagged) - Mouse Trf (TATA binding protein-related factor)-proximal protein homolog (Drosophila) (cDNA clone MGC:63299 |
CNY 2,850.00 |
|
| MR200965L1 | Lenti ORF clone of Trfp (Myc-DDK-tagged) - Mouse Trf (TATA binding protein-related factor)-proximal protein homolog (Drosophila) (cDNA clone MGC:63299 |
CNY 4,750.00 |
|
| MR200965L2 | Lenti ORF clone of Trfp (mGFP-tagged) - Mouse Trf (TATA binding protein-related factor)-proximal protein homolog (Drosophila) (cDNA clone MGC:63299 |
CNY 4,750.00 |
