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Acot13 (NM_025790) Mouse Tagged ORF Clone
CAT＃: MR200881
- TrueORF®
Acot13 (Myc-DDK-tagged) - Mouse acyl-CoA thioesterase 13 (Acot13)
ORF Plasmid: tGFP
"NM_025790" in other vectors (4)
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CNY 1,200.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 0610006O17Rik; Them2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR200881 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAGCATGACCCAGAACCTACGAGAAGTAATGAAGGTTATGTTCAAAGTTCCCGGTTTTGATAGAG TTTTGGAAAAGGTGACGCTTGTCTCGGCTGCTCCTGAGAAACTAATCTGTGAGATGAAGGTGGAAGAGCA GCATACTAATAAACTGGGCACGCTCCATGGAGGCTTGACAGCAACCTTAGTGGACAGCATCTCGACCATG GCTCTAATGTGCACAGAAAGAGGAGCACCCGGAGTCAGTGTGGACATGAACATAACGTACATGTCACCTG CTAAGATAGGAGAAGAAATAGTGATCACAGCACACATTCTGAAGCAAGGAAAGACACTTGCATTTGCCTC AGTGGATCTGACCAACAAGACCACAGGAAAATTAATAGCACAAGGCAGACACACAAAACACCTGGGGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR200881 protein sequence
Red=Cloning site Green=Tags(s) MSSMTQNLREVMKVMFKVPGFDRVLEKVTLVSAAPEKLICEMKVEEQHTNKLGTLHGGLTATLVDSISTM ALMCTERGAPGVSVDMNITYMSPAKIGEEIVITAHILKQGKTLAFASVDLTNKTTGKLIAQGRHTKHLGN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025790 |
| ORF Size | 423 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_025790.2 |
| RefSeq Size | 686 bp |
| RefSeq ORF | 423 bp |
| Locus ID | 66834 |
| UniProt ID | Q9CQR4 |
| MW | 15.2 kDa |
| Gene Summary | Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Can also hydrolyze 3-hydroxyphenylacetyl-CoA (in vitro). May play a role in controlling adaptive thermogenesis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203714 | Acot13 (untagged) - Mouse acyl-CoA thioesterase 13 (Acot13), (10ug) |
CNY 1,200.00 |
|
| MG200881 | Acot13 (tGFP-tagged) - Mouse thioesterase superfamily member 2 (Them2) |
CNY 2,850.00 |
|
| MR200881L3 | Lenti ORF clone of Acot13 (Myc-DDK-tagged) - Mouse acyl-CoA thioesterase 13 (Acot13) |
CNY 4,750.00 |
|
| MR200881L4 | Lenti ORF clone of Acot13 (mGFP-tagged) - Mouse acyl-CoA thioesterase 13 (Acot13) |
CNY 4,750.00 |
