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Leprot (NM_175036) Mouse Tagged ORF Clone
CAT＃: MR200755
- TrueORF®
Leprot (Myc-DDK-tagged) - Mouse leptin receptor overlapping transcript (Leprot)
ORF Plasmid: tGFP
"NM_175036" in other vectors (4)
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CNY 1,200.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | LEPR; OB-RGRP; Obrgrp; VPS55 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR200755 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGCGTTAAAGCTCTCGTGGCGCTCTCCTTCAGTGGGGCAATTGGGCTGACTTTTCTTATGCTGG GATGTGCGTTGGAGGACTATGGCGTTTACTGGCCCCTGTTCGTCTTGATTTTCTACGTCATCTCCCCCAT CCCCTACTTCATTGCCAAAAGGGTCACATATGACTCCGACGCGACCAGCAGTGCCTGTCGGGAACTGGCG TATTTCTTCACCACTGGGATTGTTGTTTCTGCCTTTGGACTTCCTGTTGTTCTTGCTCGTGTGGATGTGA TCAAGTGGGGAGCCTGTGGTCTTGTGCTGGCCGGCAACGCTGTCATTTTCCTCACAATTCAAGGCTTCTT CCTCGTCTTTGGAAGAGGAGATGATTTCAGTTGGGAACAGTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR200755 protein sequence
Red=Cloning site Green=Tags(s) MAGVKALVALSFSGAIGLTFLMLGCALEDYGVYWPLFVLIFYVISPIPYFIAKRVTYDSDATSSACRELA YFFTTGIVVSAFGLPVVLARVDVIKWGACGLVLAGNAVIFLTIQGFFLVFGRGDDFSWEQW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175036 |
| ORF Size | 396 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175036.4 |
| RefSeq Size | 1827 bp |
| RefSeq ORF | 396 bp |
| Locus ID | 230514 |
| UniProt ID | O89013 |
| MW | 14.3 kDa |
| Gene Summary | Negatively regulates leptin receptor (LEPR) cell surface expression, and thus decreases response to leptin. Negatively regulates growth hormone (GH) receptor cell surface expression in liver. May play a role in liver resistance to GH during periods of reduced nutrient availability.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200140 | Leprot (untagged) - Mouse leptin receptor overlapping transcript (Leprot), (10ug) |
CNY 1,200.00 |
|
| MG200755 | Leprot (tGFP-tagged) - Mouse leptin receptor overlapping transcript (Leprot) |
CNY 2,850.00 |
|
| MR200755L3 | Lenti ORF clone of Leprot (Myc-DDK-tagged) - Mouse leptin receptor overlapping transcript (Leprot) |
CNY 4,750.00 |
|
| MR200755L4 | Lenti ORF clone of Leprot (mGFP-tagged) - Mouse leptin receptor overlapping transcript (Leprot) |
CNY 4,750.00 |
